Part I: Introduction to Yeast Surface Display and Its Applications

1. Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System

Maryam Raeeszadeh-Sarmazdeh and Eric T. Boder

Part II: Construction and Selection of Yeast Surface Display Libraries and Analysis of Isolated Variants

2. Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation

Byong H. Kang, Brianna M. Lax, and K. Dane Wittrup

3. Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations

Benedikt Dolgikh and Daniel Woldring

4. Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution

James VanAntwerp, Patrick Finneran, Benedikt Dolgikh, and Daniel Woldring

5. Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries

Mehrsa Mardikoraem and Daniel Woldring

6. Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders

Nicole J. Yang

7. Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display

Preeti Sharma, Erik Procko, and David M. Kranz

8. Engineering Binders with Exceptional Selectivity

Kai Wen Teng, Akiko Koide, and Shohei Koide

9. Affinity and Stability Analysis of Yeast Displayed Proteins

Charlotte U. Zajc, Magdalena Teufl, and Michael W. Traxlmayr

Part III: Selection of Yeast Surface Display Libraries for Binding to Mammalian Cells or Extracellular Matrix

10. Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting

Stefania C. Carrara, Jan P. Bogen, Julius Grzeschik, Björn Hock, and Harald Kolmar

11. A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins

Patrick J. Krohl and Jamie B. Spangler

12. Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques

Rojhae A. Panton and Lawrence A. Stern

13. Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display

Benjamin J. Umlauf, John S. Kuo, and Eric V. Shusta

Part IV: Specialized Yeast Surface Display Applications

14. Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology