1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments
Douglas W. Houston
2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos
Thao Nguyen, Timothy J. Mitchison, and Martin Wühr
3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos
Michael D. Sheets
4. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts
Alexander A. Tokmakov and Ken-Ichi Sato
5. Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis
Ken-ichi Sato and Alexander A. Tokmakov
6. Embryological and Genetic Manipulation of Chick Development
Laura S. Gammill, Bridget Jacques-Fricke, and Julaine Roffers-Agarwal
7. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos
Ana Paula Azambuja and Marcos Simoes-Costa
8. Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens
Triveni Menon and Sreelaja Nair
9. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)
Karoline Holler and Jan Philipp Junker
10. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos
Clare E. Buckley
11. Ex Utero Culture and Imaging of Mouse Embryos
Sonja Nowotschin, Vidur Garg, Anna Piliszek, and Anna-Katerina Hadjantonakis
12. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections
Edwina McGlinn, Miriam Holzman, and Jennifer H. Mansfield
13. Reptile Embryology and Regeneration
Matthew K. Vickaryous and Emily A.B. Gilbert
14. Manipulation of Developmental Function in Turtles with Notes on Alligators
Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, and Judith A. Cebra-Thomas
15. Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes
Amanda Butler, Dawn Owens, Mary Lou King, and Tristan Aguero
16. Visualizing the Balbiani Body in Zebrafish Oocytes
KathyAnn L. Lee and Florence L. Marlow
17. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis
Allison Jamieson-Lucy and Mary C. Mullins
18. Generation of
