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Untersuchungen zur Wirkung von KP 735 auf das klonogene Wachstum maligner frisch explantierter humaner Tumorzellen in vitro

Untersuchungen zur Wirkung von KP 735 auf das klonogene Wachstum maligner frisch explantierter humaner Tumorzellen in vitro

          
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About the Book

Doktorarbeit / Dissertation aus dem Jahr 1997 im Fachbereich Medizin - Neoplasmen, Onkologie, Note: 2, Technische Universität München, Sprache: Deutsch, Abstract: Im Rahmen der Dissertationsarbeit wurden Untersuchungen zur Wirkung von cis-Diammin-[(bis(phosphonatomethyl)amino)acetato(2-)-O1, N1]platin(II) (KP 735) auf das klonogene Wachstum maligner frisch explantierter humaner Tumorzellen in vitro durchgeführt. KP 735 ist eine technisch synthetisierte, phosphonsäure-gekoppelte Platinverbindung. Die antiproliferative Wirkung von KP 735 auf die Kolonienbildung von 74 frisch explantierten humanen Primärtumoren wurde mittels eines für klonogene Zellen selektiven kapillären Weichagarklonierungsverfahrens untersucht. Die Endkonzentrationen von KP 735 betrugen 10,0 µg/ml, 1,0 µg/ml und 0,1 µg/ml in der Langzeitexposition, sowie 100,0 µg/ml, 10,0 µg/ml, 1,0 µg/ml, 0,4 µg/ml, 0,32 µg/ml, und 0,1 µg/ml in der 1h-Exposition. In der kontinuierlichen Exposition waren 53 von 74 (72%) auswertbaren Proben ausreichend gewachsen, in der Kurzzeitexposition 51 von 74 (69%). KP 735 hemmte das klonogene Wachstum der Tumorzellen Konzentrations- und Expositionszeit-abhängig. In der Langzeitexposition wurden 32 von 53 Proben (60%) bei 10,0 µg/ml, 10 von 53 (19%) bei 1,0 µg/ml und 1 von 53 (2%) bei 0,1 µg/ml KP 735 gehemmt. In der Kurzzeitexposition hemmte KP 735 in einer Konzentration von 100,0 µg/ml 30 von 51 (59%), bei 10,0 µg/ml 15 von 51 (29%), bei 1,0 µg/ml 6 von 51 (12%), bei 0,40 µg/ml 3 von 51 (6%), bei 0,32 µg/ml 0 von 51 (0%), bei 0,10 µg/ml 1 von 51 (2%) der Proben. Cis-Diammin-[(bis(phosphonatomethyl)amino)acetato(2-)-O1, N1]platin(II), (KP 735) is a new platinum based agent wich has shown antitumor activity in established tumor cell lines. We have studied the effects of KP 735 on soft agar colony formation of freshly explanted human tumors in vitro and found antineoplastic activity.


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Product Details
  • ISBN-13: 9783640331055
  • Publisher: Grin Publishing
  • Binding: Paperback
  • Language: German
  • Returnable: N
  • Spine Width: 7 mm
  • Width: 148 mm
  • ISBN-10: 3640331052
  • Publisher Date: 09 Jun 2009
  • Height: 210 mm
  • No of Pages: 112
  • Series Title: German
  • Weight: 154 gr


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