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Bacteria under stress. How bacteria perceive and process information

Bacteria under stress. How bacteria perceive and process information

          
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About the Book

Project Report from the year 2014 in the subject Biology - Micro- and Molecular Biology, grade: 2.0, LMU Munich, language: English, abstract: The analysis of stress response systems in microorganisms can reveal molecular strategies for regulatory control and adaptation. For example, external pH implicates as a signal in growing number of genetic and molecular responses in enteric bacteria like Escherichia coli. In number of cases, acid-induced gene expression functions to decrease the acidity of bacterial products in an acidic environment. Thus, enhancing the growth at a low pH. In this experiment, we examine the gene expression of the E. coli cadBA operon. The cad operon consists of the enzyme CadA, which is a lysine decarboxylase, the transport protein CadB and the regulatory protein CadC. The cad operon is active when there is a low pH and lysine in the periplasm of bacteria cells. The pH sensor CadC, which is located in the periplasm, recognizes the low pH and lysine. As a result, DNA can bind and activate the transcription, so that there can be an expression of the two downstream genes cadB and cadA. Furthermore, the CadA enzyme helps to produce cadaverine. The reaction of lysine to cadavarine effectively consumes protons (H⁺) and lead to an increase of the internal pH. Moreover, the antiporter CadB imports the substrate lysine and exports its product cadaverine. Together, they reduce the intracellular H⁺ concentration. CadC is a member of the ToxR family and can measure the external amount of cadaverine. CadC consists of a sensor domain which is monitoring the extracellular pH and cadavarine; a transmembrane domain which interacts with LysP (Co-sensor for lysine) and the effector domain, which is a DNA-binding domain. CadC can inhibit the transcription of CadA. When the amount of cadaverine increases in the bacterial cell, the cad operon switches off of producing cadaverine by a signalling molecule called ppGpp. Under acidic conditions, ppGpp can bind


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Product Details
  • ISBN-13: 9783668523081
  • Publisher: Bod Third Party Titles
  • Publisher Imprint: Grin Verlag
  • Height: 210 mm
  • No of Pages: 32
  • Spine Width: 2 mm
  • Width: 148 mm
  • ISBN-10: 3668523088
  • Publisher Date: 20 Sep 2017
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 54 gr


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