PART I: Overview Chapters1. AlloreactivitySidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey
2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury
Nicole M. Valenzuela and Elaine F. Reed
3. Cell Mediated Rejection
Kathryn J Wood, Masaaki Zaitsu and Ryoichi Goto
4. Transplantation Tolerance
Colin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg
5. Composite Tissue Transplantation
Gerald Brandacher
6. Transplantation of the Sensitized Patient: Histocompatibility Testing
Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary
7. Thoracic Organ Transplantation: Laboratory Methods
Jignesh K. Patel and Jon A. Kobashigawa
PART II: Methods Chapters
8. HLA Typing by Sequence Specific Primers
Mike Bunce, Biofortuna and Ben Passey, Biofortuna
9. Human Leukocyte Antigen (HLA) Typing By DNA Sequencing
Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley
10. Next-Generation HLA Sequencing using the 454 GS FLX System
Elizabeth A. Trachtenberg and Cherie L. Holcomb
11. Chimerism Testing by Quantitative PCR Using Indel Markers
Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer
12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR)
Raja Rajalingam and Elham Ashouri
13. Complement-dependent Cytotoxicity Crossmatch
Jeremy Ryan Peña, Donna Fitzpatrick, and Susan L. Saidman
14. Lymphocyte Crossmatching by Flow Cytometry
Robert A. Bray
15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies
John D Smith and Marlene L Rose
16. C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens
Ge Chen and Dolly B. Tyan
17. Tetramer Staining for the Detection of HLA-specific B cells
Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary
18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood
Annette M. Jackson, Donna P. Lucas, Jessica L. Badders
19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation
Duska Dragun
20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA)
Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar
21. Cylex ImmuKnow Cell Function Assay
Adriana Zeevi and John Lunz
22. Detection of Intracellular Cytokines
Nancy L. Reinsmoen and Chih-Hung Lai
23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients
George Vlad and Nicole Suciu-Foca
24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA
Tara K. Sigdel, and Minnie. M. Sarwal
25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing
Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian
