Contents

Preface

Contributors

Part I. Selection AND DETECTION methods

1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation

Craig M. Richael and Caius M. Rommens

2. Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed

T. Scott Pinkerton, James R. Wild, and John A. Howard

3. Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants

Basel Khraiwesh

Part II. Transformation Technology - Lower Plants

4. Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock

Part III. Transformation Technology - Rice

5. A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.)

Kenjirou Ozawa

6. Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene

Masaki Endo, Tsutomu Shimizu and Seiichi Toki

7. Visual Selection in Rice-a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products

Hiroaki Saika, Haruko Onodera and Seiichi Toki

8. Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System

Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui

9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments

Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui

Part IV. Transformation Technology - Cereals and Other Monocots

10. Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos

Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux

11. Split-transgene Expression in Wheat

Mario Gils, Myroslava Rubtsova, and Katja Kempe

12. Agrobacterium-mediated Transformation of Brachypodium Distachyon

Vera Thole and Philippe Vain

13. Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules

Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm

14. Biolistic Mediated Production of Transgenic Oil Palm

Ghulam Kadir Ahmad Parveez and Bohari Bahariah

15. Transformation of Oil Palm using Agrobacterium tumefaciens

Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani

Part V. Transformation Technology - Dicots

16. Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)

César Petri, Ralph Scorza, and Chinnathambi Srinivasan

17. Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes

Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray

18. Initiation and Transformation of Grapevine Embryogenic Cultures

Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray

19. Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless

Mercedes Dabauza and Leonardo Velasco