Part I: Toll-Like Receptor Detection and Activation

1. Toll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action
Jennifer K. Dowling and Jérome Dellacasagrande

2. Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures
Tom P. Monie, Nicholas J. Gay, and Monique Gangloff

3. Toll-Like Receptor Interactions Measured by Microscopic and Flow Cytometric FRET
Gabor L. Horwath, Pia Langhoff, and Eicke Latz

4. Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors
Harald Husebye and Sarah L. Doyle

5. Assessing the Inhibito

Part II: Toll-Like Receptor Cross-Priming of Associated Receptors

6. Methods for Delivering DNA to Intracellular Receptors
Katryn J. Stacey, Adi Idris, Vitaliya Sagulenko, Nazarii Vitak, and David P. Sester

7. Detection of Interaction between Toll-Like Receptors and Other Transmembrane Proteins by Co-Iummunoprecipitation Assay
Yu-Ran Lee, Won Dae Kang, and You-Me Kim

8. Flow Cytometry-Based Bead-Binding Assay for Measuring Receptor Ligand Specificity
Joris K. Sprokholt, Nina Hertoghs, and Tennis B.H. Geijtenbeek

9. Measuring Monomer-to-Filament

Part III: Toll-Like Receptor Post-Transcriptional Regulation

10. Co-Transcriptomic Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression in Host and Bacterial Pathogen
Timothy Ravasi, Charalampos (Harris) Mavromatis, Nilesh J. Bokil, Mark A. Schembri, and Matthew J. Sweet

11. Simple Methods to Investigate MicroRNA Induction in Response to Toll-Like Receptors
Victoria G. Lyons and Claire E. McCoy

12. Determining the Function of Long Noncoding RNA in Innate Immunity
Susan Carpenter

13. Analysis of Post-Transcriptional Gene Regulation of Nod-Like Receptors via the 3'UTR
Moritz Haneklaus

Part IV: Toll-Like Receptors and System Control

14. TLR Function in Murine CD4+ T Lymphocytes and their Role in Inflammation
Stephanie Flaherty and Joseph M. Reynolds

15. Flow Cytometry Analysis of B Cell Activation and Antibody Responses Induced by Toll-Like Receptors
Egest J. Pone

16. Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells
Krishna L. Moody, Melissa B. Uccellini, Ana M. Avalos, Ann Marshak-Rothstein, and Gregory A. Viglianti

17. Analysis of TLR-Induced Metabolic Changes in Dendritic Cells U

18. Toll-Like Receptor Signaling and the Control of Intestinal Barrier Function
Daniel G.W. Johnston and Sinéad C. Corr

19. Understanding the Role of Cellular Molecular Clocks in Controlling the Innate Immune Response
Anne M. Curtis and Caio T. Fagundes

Part V: Toll-Like Receptors and Disease

20. Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis
Marc Schmidt, Matthias Goebeler, and Stefan F. Martin

21. Allergens and Activation of the Tol

22. Investigating the Role of Toll-Like Receptors in Models of Arthritis
Anna M. Piccinini, Lynn Williams, Fiona E. McCann, and Kim. S. Midwood

23. Delineating the R