Part I. mRNA Synthesis, Purification, and Transfer
1. Cell Engineering with Synthetic Messenger RNA
Peter M. Rabinovich and Sherman M. Weissman
2. In vitro Transcription of Long RNA Containing Modified Nucleosides
Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karikó
3. HPLC Purification of in vitro Transcribed Long RNA
Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karikó
4. Synthetic mRNAs with Superior Translation and Stability Properties
Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads
5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA
James G. Hecker
6. Light-Induced mRNA Transfection
Sigurd Leinæs Bøe and Eivind Hovig
7. Engineering B Cells with mRNA
Jaewoo Lee, David Boczkowski, and Smita Nair
8. Programming Human Dendritic Cells with mRNA
Jaewoo Lee, David Boczkowski, and Smita Nair
9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process
Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa
10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation
Melanie Galla, Axel Schambach, and Christopher Baum
11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells
Frank Breinig, Tanja Breinig, and Manfred J. Schmitt
Part 2. Modulation and monitoring of cell metabolism
12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy
Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken
13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors
Noriko Shimasaki and Dario Campana
14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA
Peizhe Wang and Jie Na
15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies
Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin
16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA
Chantal Pichon and Patrick Midoux
17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA
Özcan Met and Inge Marie Svane
18. mRNA electroporation as a Tool for Immunomonitoring
Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman
19. mRNA PCR-Based Epitope Chase Method
Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe
