1. What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs
Olga Matveeva
2. Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques
Shigeru Takasaki
3. Designing Functional siRNA with Reduced Off-Target Effects
Yuki Naito and Kumiko Ui-Tei
4. Design and Screening of siRNAs Against Highly Structured RNA Targets
Neda Nasheri, John Paul Pezacki, and Selena M. Sagan
5. Engineering Small Interfering RNAs by Strategic Chemical Modification
Jesper B. Bramsen and Jørgen Kjems
6. The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs
Narendra Vaish and Pinky Agarwal
7. The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells
Chanil Chang, Sun Woo Hong, Pooja Dua, Soyoun Kim, and Dong-ki Lee
8. Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA)
Elena L. Chernolovskaya and Marina A. Zenkova
9. Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting
Pål Sætrom
10. Strategies for Designing and Validating Immunostimulatory siRNAs
Michael P. Gantier
11. Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs)
Vineeth Surendranath, Mirko Theis, Bianca H. Habermann, and Frank Buchholz
12. Short Hairpin RNA-Mediated Gene Silencing
Luke S. Lambeth and Craig A. Smith
13. Design of Lentivirally Expressed siRNAs
Ying Poi Liu and Ben Berkhout
14. Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application
Donald D. Rao, Neil Senzer, Zhaohui Wang, Padmasini Kumar, Chris M. Jay, and John Nemunaitis
15. Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers
Anne Dallas and Brian H. Johnston
16. Production and Application of Long dsRNA in Mammalian Cells
Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda
17. Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors
Thomas Horn and Michael Boutros
18. Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase
Hiromitsu Tanaka
19. Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses
Muhammad Fahim and Philip J. Larkin
20. Downregulation of Plant Genes with MiRNA Induced Gene Silencing
Felipe Fenselau de Felippes
