1. Top 5 Considerations When Choosing an Antibody
Amy Archuleta and Michael D. Browning
2. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting
Biji T. Kurien and R. Hal Scofield
3. Recent Advances in Chromogens for Immunohistochemistry
Bipin Gupta, George Yang, Olga Petrauskene, and Marc Key
4. Overview of Imaging Techniques in Immunohistochemistry
Gerald Sedgewick
5. Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells
Pravin J. Mishra and Debabrata Banerjee
6. Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments
Hemant K. Mishra
7. Labeling of Phospho-Specific Antibodies with oYo-Link(R) Epitope Tags for Multiplex Immunostaining
Jingwen Niu, Jodi Hagen, Feifan, Yu, Alexander E. Kalyuzhny, and Andrew Tsourkas
8. Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species
Matthew Levin, Zohreh AkhavanAghdam, and David Schwartz
9. 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes
Sharla L. White, Amy T. Lam, and Hannah D. Buck
10. Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research
Simeon C. Daeschler and Gregory H. Borschel
11. Protocol and Software for Automated Detection of Lysosome Active "Runs" and "Flights" with Wavelet Transform Approach
Kristiana Kandere-Grzybowska, Konstantin Polev, Diana V. Kolygina, and Bartosz A. Grzybowski
12. Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing
Christine Goetz, Chris Hammerbeck, Kristina Boss, Ryan Peterson, and Jody Bonnevier
13. In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells
Jodi Hagen, Ashley Oliver, and Alexander E. Kalyuzhny
14. In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue
Ashley Oliver, Jodi Hagen, Sue Yang, and Alexander E. Kalyuzhny
15. Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry
Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan, Murray J. Tipping, Afsar Barlas, Yevgeniy Romin, and Katia Manova-Todorova
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