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RNAi Technologies

RNAi Technologies

          
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About the Book

RNA interference (RNAi), one of the most exciting discoveries of the last decade, is also known as Post-Transcriptional Gene Silencing (PTGS). It is a conserved host defense response via double-stranded RNA against both endogenous and foreign nucleic acids, and occurs at the levels of transcription, post-transcription and translation. RNA induced gene silencing has been used, with considerable success as a reverse genetics tool for the study of gene function. This technology has revolutionized the gene expression analysis studies because of the specificity and speed it offers. The small RNA molecules central to RNAi are microRNA (miRNA) and small interfering RNA (siRNA). These molecules bind to other RNAs specifically, such as messenger RNA (mRNA), to regulate their activities.In this book, we will discuss how gene silencing methodology has been successfully applied as therapeutic tool against metabolic disorders, cancer, and infectious diseases. This tool has also been successfully used to improve crops resistance against plant pathogens. In contrast to conventional knock-out mutagenesis techniques, the "gene-silencing" vectors, that allow the study of functions of genes whose complete knock-out would otherwise be lethal to the plant.
About the Author: Dr Prerna Pandey, a biotechnologist with several years of wet lab research experience, has worked as a senior research fellow at International Center for Genetic Engineering and Biotechnology, New Delhi. Her field of research in PhD included isolation and molecular characterization of Geminiviruses, genome sequencing, gene annotation, as well as gene silencing using the RNA interference technology. She has published papers in peer-reviewed journals in the field and has a submitted a number of annotated Geminiviral genome sequences in the GenBank, including two novel ones. Prerna has also completed her editing and proof-reading courses from SfEP, UK and now works as a freelance scientific editor, writer and a subject matter expert. She has edited and co-authored several books on a range of topics in Life-Sciences. When Prerna is not busy with her assignments, she loves visiting historical places.


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Product Details
  • ISBN-13: 9781773611471
  • Publisher: Delve Publishing
  • Publisher Imprint: Delve Publishing
  • Height: 229 mm
  • No of Pages: 377
  • Spine Width: 0 mm
  • ISBN-10: 177361147X
  • Publisher Date: 01 Nov 2017
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Width: 152 mm


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