1. Designing Hairpin-Based RNAi Shuttles
Yu Shen
2. Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide
Ryan L. Boudreau, Sara E. Garwick-Coppens, Jian Liu, Lindsay M. Wallace, and Scott Q. Harper
3. The Use of Small Non-Coding RNAs to Silence Transcription in Human Cells
Kevin V. Morris
4. Delivery Strategies for RNAi to the Nervous System
Kevin D. Foust and Brian K. Kaspar
5. Cloning Small RNAs
Eric J. Devor and Lingyan Huang
6. Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays
Peter White
7. High Throughput Profiling of Mature MicroRNA by Real-Time PCR
Jinmai Jiang, Eun Joo Lee, Melissa Piper Hunter, Clay B. Marsh, and Thomas D. Schmittgen
8. In Situ Detection of MicroRNAs in Paraffin Embedded, Formalin Fixed Tissues: Different Methodologies and Co-Localization with Possible Targets
Gerard J. Nuovo, Patrick Nana-Sinkam, Melissa Crawford, and Thomas D. Schmittgen
9. Tracing of siRNAs Inside Cells by FRET Imaging
Markus Hirsch, Il-Han Kim, Anne Järve, Roger Fischer, Michael F. Trendelenburg, Ulrich Massing, Karl Rohr, and Mark Helm
10. Application of RIP-Chip for the Identification of miRNA Targets
Lu Ping Tan, Anke van den Berg, and Joost L. Kluiver
