Part I: Review

1. Ribosome Display: A Perspective

Andreas Plückthun

Part II: Translation Extract Preparation

2. Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts

James F. Zawada

Part III: Basic Ribosome Display and Related Selection Methods

3. Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate

Julie A. Douthwaite

4. Stabilized Ribosome Display for In Vitro Selection

Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li, and Yoshihiro Ito

5. Eukaryotic Ribosome Display with In Situ DNA Recovery

Mingyue He, Bryan M. Edwards, Damjana Kastelic, and Michael J. Taussig

6. mRNA Display Using Covalent Coupling of mRNA to Translated Proteins

Rong Wang, Steve W. Cotten, and Rihe Liu

7. SNAP Display: In Vitro Protein Evolution in Microdroplets

Miriam Kaltenbach and Florian Hollfelder

8. cDNA Display: Rapid Stabilization of mRNA Display

Shingo Ueno and Naoto Nemoto

Part IV: Applications of Ribosome Display Methods Using Natural Amino Acids

9. Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis

Leeanne Lewis and Chris Lloyd

10. Affinity Maturation of Phage Display Antibody Populations using Ribosome Display

Maria A. Groves and Adrian A. Nickson

11. Evolution of Protein Stability Using Ribosome Display

Andrew Buchanan

12. Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries

Peter Ravn

13. Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display

Yuki Mochizuki and Naoto Nemoto

14. Peptide Screening Using PURE Ribosome Display

Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K. Akbar, and Takuya Ueda

15. Rapid Selection of High Affinity Binders Using Ribosome Display

Birgit Dreier and Andreas Plückthun

16. mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries

Steve W. Cotten, Jianwei Zou, Rong Wang, Bao-cheng Huang, and Rihe Liu

17. Identification of Candidate Vaccine Genes Using Ribosome Display

Liancheng Lei

18. Ribosome Display for the Selection of Sac7d Scaffolds

Barbara Mouratou, Ghislaine Béhar, Lauranne Paillard-Laurance, Stéphane Colinet, and Frédéric Pecorari

Part V: Incorporation of Non-Natural Amino Acids for Selection by Ribosome Display and Related Methods

19. Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers

Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki Suga

20. Update on Pure Translation Display with Unnatural Amino Acid Incorporation

R. Edward Watts and Anthony C. Forster

21. In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display

Zhong Ma and Matthew C.T. Hartman

Part VI: Case Studies

22. Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display

George Thom and Ralph Minter