A broad range of new properties is emerging from supramolecular aggregates. Self-assembled structures of purely peptidic amphiphiles exploit these properties to produce biocompatible, biodegradable, smart materials for drug administration. This thesis explores the design, synthesis, purification, characterization of purely peptidic amphiphiles, and the evaluation of potential applications. The first chapter provides a general introduction to the field of self-assembly and drug delivery as compared to nature’s delivery mechanisms. Further, the advantage of amino acid based molecules in producing smart materials for drug delivery applications is highlighted via biocompatibility and biodegradability considerations. Next, synthetic strategies and purification methods are discussed. Finally gramicidin A (gA) – a naturally occurring, short, hydrophobic, membrane-integrating peptide used to produce the amphiphilic peptides presented here – is introduced. Chapter two presents an initial approach to produce self-assembled structures by purely peptidic amphiphiles. The undecamer used features a repetitive L-tryptophan and D-leucine [LW-DL] motif representing the hydrophobic block, and an N-terminally attached hydrophilic (lysine or acetylated lysine) section. Besides solid-phase peptide synthesis and purification, the process that self-assembles micelles and spherical peptide particles, termed “peptide beads” was characterized as a function of temperature and solvent composition by means of electron paramagnetic resonance (EPR), dynamic and static light scattering, fluorimetry and electron microscopy. An equilibrium process between single peptide molecules, micelles and peptide beads is then presented. Chapter three examines the structure of self-assembled peptide beads of diameters between 200 to 1500 nm. The beads were analyzed by electron and atomic force microscopy (AFM), static and dynamic light-, and small angle X-ray scattering. The beads are seen to result from hierarchical organization of micellar-like subunits and confirm the concept of multicompartment micelles. An improved understanding of the beads’ capacity to embed hydrophobic and hydrophilic payloads and provide perspectives for drug delivery applications emerges. Chapter four presents a library of longer peptides, based on the full sequence of gA. The peptide design includes three (a) a charged lysine part, (b) an acetylated lysine part and (c) a constant hydrophobic rod-like helix, based on gA. Stepwise replacement of free lysine (K) with acetylated lysine (X) generated the ten peptides Ac-X8-gA and KmX8-m-gA (m ranging from 0 to 8). With the change in the primary sequence, a change in secondary structure was observed. The transition reflected a change in the self-assembled structures from fibers to micelles. This demonstrates how even small point mutations influence the supramolecular outcome and serve as an important step to understanding and controlling self-assembly. In chapter five, the knowledge gained on gA-based peptides is applied to produce purely peptidic vesicles. The work here demonstrated that, to form such structures with short amphiphiles, additional stabilizing factors were necessary. Thus, we exploited different dimerization strategies to form stable peptide membranes and developed a general recipe to form purely peptidic vesicles. The vesicles demonstrated pH responsiveness as well as the capacity to embed hydrophilic and hydrophobic payloads in their structure. Chapter six presents the potential of self-assembled peptide beads in drug delivery applications. The hydrophobic and hydrophilic payload-filled peptide beads are shown to be internalized by human cells. Further, a method to increase embedding efficiency for RNA/DNA to 99% due to charge-driven complexation and embedding is presented. The internalization of the gene delivery vehicle into cells led to gene sile