Part I: Protein Biophysics Assays
1. Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation
Joana S. Cristóvão, Bárbara J. Henriques, and Cláudio M. Gomes
2. Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, α-Synuclein, and Prion Aggregates
Eri Saijo, Bradley R. Groveman, Allison Kraus, Michael Metrick, Christina D. Orrù, Andrew G. Hughson, and Byron Caughey
3. Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides
Ravit Malishev, Sofiya Kolusheva, and Raz Jelinek
4. Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis
Naoto Soya, Ariel Roldan, and Gergely L. Lukacs
5. A Multipronged Method for Unveiling Subtle Structural-Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies
Donatella Bulone, Pier Luigi San Biagio, Tatiana Quiñones-Ruiz, Manuel Rosario-Alomar, Igor K. Lednev, Frank T. Robb, Everly Conway de Macario, and Alberto J.L. Macario
6. High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1
Alireza Abdolvahabi, Sanaz Rasouli, Corbin M. Croom, and Devon L. Plewman
7. Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases
Devkee M. Vadukul, Youssra K. Al-Hilaly, and Louise C. Serpell
8. Assays for Light Chain Amyloidosis Formation and Cytotoxicity
Luis M. Blancas-Mejia, Pinaki Misra, Christopher J. Dick, Marta Marin Argany, Keely R. Redhage, Shawna A. Cooper, and Marina Ramirez-Alvarado
Part II: Cellular And Proteostasis Assays
9. Monitoring Aggregate Clearance and Formation in Cell-Based Assays
Evelien Eenjes, Young Joo Yang-Klingler, and Ai Yamamoto
10. Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor
Yu Liu, Matthew Fares, and Xin Zhang
11. Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation
Vibha Prasad and Aaron Voigt
12. Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry
Salvador Ventura and Susanna Navarro
13. Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells
Edward Pokrishevsky, Jeremy Nan, and Neil R. Cashman
14. A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction
Cagla Cömert, Paula Fernandez Guerra, and
