Part I: Overview Articles
1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development
Philip Kirk and Yoselin Benitez-Alfonso
2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses
Caiping Huang and Manfred Heinlein
Part II: Structure and Distribution of Plasmodesmata
3. Plasmodesmata Ultrastructure Determination Using Electron Tomography
Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer
4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata
Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska
5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots
Andrea Paterlini and Ilya Belevich
6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities
Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith
7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy
Florence R. Danila
8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy
Kirsten Knox
Part III: Compositional Analysis of Plasmodesmata
9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata
Caiping Huang, Jerôme Mutterer, and Manfred Heinlein
10. Immunofluorescence Detection of Callose in Plant Tissue Sections
Sam Amsbury and Yoselin Benitez-Alfonso
11. Callose Detection and Quantification at Plasmodesmata in Bryophytes
Arthur Muller, Tomomichi Fujita, and Yoan Coudert
12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis
Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison
13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons
Verena Kriechbaumer and Stanley W. Botchway
14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer Patrick Blümke, Vicky Howe, and Rüdiger Simon
15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy
Joseph F. McKenna
16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering
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