Part I: Review and Overview Chapters
1. Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
Kerstin Kaufmann and Chiara A. Airoldi
2. Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
Felix Wolter and Holger Puchta
3. The Long-Term 'In Natura' Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
Hiroshi Kudoh, Mie N. Honjo, Haruki Nishio, and Jiro Sugisaka
Part II: Preparation of Plant Materials
4. Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
Pooja Aggarwal, Krishna Reddy Challa, Monalisha Rath, Preethi Sunkara, and Utpal Nath
5. A Specific Knockdown of Transcription Factor Activities in Arabidopsis
Beibei Zheng, Bennett Thomson, and Frank Wellmer
6. Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
Ze Hong Lee, Nobutoshi Yamaguchi, and Toshiro Ito
7. CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
Shigeo S. Sugano and Ryuichi Nishihama
8. Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
Tatyana Radoeva, Shunsuke Saiga, and Dolf Weijers
9. Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells Kyohei Uemoto, Takashi Araki, and Motomu Endo
10. Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
Alif Meem Nurani, Yuki Kondo, and Hiroo Fukuda
Part III: Detection of Expression Levels
11. High Impact Gene Discovery: Simple Strand Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
Yasunori Ichihashi, Atsushi Fukushima, Arisa Shibata, and Ken Shirasu
12. Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
Brice Roux, Nathalie Rodde, Sandra Moreau, Marie-Françoise Jardinaud, and Pascal Gamas
13. nanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
Maria G. Ivanchenko and Molly Megraw
14. Genome-Wide TSS Identification in Maize
María Katherine Mejia-Guerra, Wei Li, Andrea I. Doseff, and Erich Grotewold
15. Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
&nb
