1. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations
Daryl J.V. David, Melanie A. McGill, R.F. Andrew McKinley, and Tony J.C. Harris
2. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell
Bertrand Jauffred and Yohanns Bellaiche
3. Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus
Yingqun Wang
4. Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity
David Neff, Justin Hogan, and Simon Collier
5. Analysis of Cell Shape and Polarity during Zebrafish Gastrulation
Douglas C. Weiser and David Kimelman
6. Analyzing Planar Cell Polarity during Zebrafish Gastrulation
Jason R. Jessen
7. Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation
Gun-Hwa Kim, Edmond Changkyun Park, and Jin-Kwan Han
8. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling
Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, and Ira O. Daar
9. Gene Loss-of-Function and Live Imaging in Chick Embryos
Anne C. Rios, Christophe Marcelle, and Olivier Serralbo
10. Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation
Courtney Mezzacappa, Yuko Komiya, and Raymond Habas
11. Convergent Extension Analysis in Mouse Whole Embryo Culture
Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D.E. Greene, and Andrew J. Copp
12. Analysis of PCP Defects in Mammalian Eye Lens
Yuki Sugiyama and John W. McAvoy
13. Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea
Helen May-Simera and Matthew W. Kelley
14. Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells
Lisa Fujimura and Masahiko Hatano
15. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration
Kristi LaMonica and Laura Grabel
16. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells
Mitsuharu Endo, Michiru Nishita, and Yasuhiro Minami
17. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling
Shinji Matsumoto and Akira Kikuchi
18. The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity
Makoto Matsuyama and Akihiko Shimono
19. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models
Daniel J. Jagger and Andrew Forge
20. Morphometric Analysis of Centrosome Position in Tissues
Hester Happé, Emile de Heer, and Dorien J.M. Peters
