PCR - Polymerase-Kettenreaktion

PCR - Polymerase-Kettenreaktion

          
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About the Book

Keine molekularbiologische Technik hat einen derart groAen Einfluss auf die Erforschung des menschlichen Genoms sowie auf das VerstAndnis der molekularen Mechanismen von genetisch bedingten Krankheiten wie die ((b))Polymerase-Kettenreaktion((/b)) (PCR). Sie ermAglicht die rasche VervielfAltigung sehr geringer NucleinsAurenmengen aus den unterschiedlichsten Ausgangsmaterialien. Ziel dieses handlichen, deutschsprachigen Laborhandbuches ist es, die Standard- und Spezialanwendungen der PCR in praxisnaher und verstAndlicher Form darzustellen. Es wendet sich an Diplomanden, Doktoranden, Wissenschaftler und technische AssistentInnen, die die MAglichkeiten der PCR fA1/4r ihre molekularbiologischen oder diagnostischen Fragestellungen nutzen mAchten. Nach einer kurzen EinfA1/4hrung in die Grundlagen der PCR werden die verschiedenen Applikationen in einzelnen Kapiteln dargestellt, von denen jedes mit der ErlAuterung beginnt, warum die spezifische Methode A1/4berhaupt eingesetzt wird, welche Materialien erforderlich sind und mit welchen Fehlern gerechnet werden muss. Dann werden die einzelnen Anwendungen Schritt fA1/4r Schritt in Form von sog. "Man-nehme"-Vorschriften dargeboten. Die zu erwartenden Ergebnisse werden in Form von Grafiken, Gelbildern und/oder Schemata veranschaulicht. Neben den eigentlichen PCR-Vorschriften werden auch Methoden zur Reinigung, Klonierung und Modifikation der PCR-Produkte beschrieben. Das Werk schlieAt mit einem ausfA1/4hrlichen Lieferantenverzeichnis.


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Product Details
  • ISBN-13: 9783827410580
  • Publisher: Spektrum Akademischer Verlag
  • Publisher Imprint: Spektrum Akademischer Verlag
  • Language: German
  • Returnable: N
  • Weight: 0 gr
  • ISBN-10: 3827410584
  • Publisher Date: 07 Nov 2018
  • Binding: Book
  • No of Pages: 134
  • Series Title: German


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