1. In silico PCR Tools for a Fast Primer, Probe and Advanced Searching Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin
2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis
Ruslan Kalendar and Timofey V. Tselykh
3. Long Fragment Polymerase Chain Reaction
Eng Wee Chua, Simran Maggo, and Martin A. Kennedy
4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR
Maria Jorge Campos and Alberto Quesada
5. Inverse PCR for Point Mutation Introduction
Diogo Silva, Gustavo Santos, Mário Barroca, and Tony Collins
6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR
Tatiana Q. Aguiar, Carla Oliveira, and Lucília Domingues
7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli
Ana Filipa Sequeira, Joana L.A. Brás, Vânia O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes
Flávio Azevedo, Humberto Pereira, and Björn Johansson
9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water
Luc Bissonnette, Andrée F. Maheux, and Michel G. Bergeron
10. Multiplex Detection Of Food-borne Pathogen
Germán Villamizar-Rodríguez and Felipe Lombó
11. Fast Real-time PCR for the Detection of Crustacean Allergens in Foods Francisco J. Santaclara and Montserrat Espiñeira
12. Fast Real-time PCR Method for the Detection of Soy in Foods
Montserrat Espiñeira and Francisco J. Santaclara
13. RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products
Joana Cunha and Lucília Domingues
14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers
Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma
15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism
Laura Vilarinho and Célia Nogueira
16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities
S.S. Phadke, A.F. Salvador, J.I. Alves, O. Bretschger, M.M. Alves, and M.A. Pereira
17. PCR in Metagenomics
Sarita G Bhat and Tina K. J.
18. Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them Leigh Burgoyne, Lin Y Koh, and David Catcheside
