1. Real-time Quantitative PCR, Pathogen Detection and MIQE
Jamie Murphy, Tania Nolan and Stephen A. Bustin
2. Overcoming Inhibition in Real-time Diagnostic PCR
Johannes Hedman and Peter Rådström
3. Quality in the Molecular Microbiology Laboratory
Paul S. Wallace and William G. MacKay
4. Pre-Analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood
Wendy L. J. Hansen, Cathrien A. Bruggeman, and Petra F. G. Wolffs
5. Detection of Bacterial Contamination in Platelet Concentrates using Flow Cytometry and Real-time PCR Methods
Tanja Vollmer, Knut Kleesiek, and Jens Dreier
6. Multiplex Real-time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton-Valentine Leukocidin from Clinical Samples
Lynne Renwick, Anne Holmes, and Kate Templeton
7. PCR Detection of Haemophilus influenzae from Respiratory Specimens
Guma M. K. Abdeldaim and Björn Herrmann
8. Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-time PCR
Clare L. Ling and Timothy D. McHugh
9. Use of Multiple Real-time PCR Assays for the Diagnosis of Bodetella spp in Clinical Specimens
Kathleen M. Tatti and M. Lucia Tondella
10. Detection of Mycoplasma pneumoniae by Real-time PCR
Jonas M. Winchell and Stephanie L. Mitchell
11. Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia
Judith Fillaux and Antoine Berry
12. Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens
Wing-Cheong Yam and Kit-Hang Gilman Siu
13. Direct Detection of Mycobaterium ulcerans in Clinical Specimens and Environmental Samples
Caroline J. Lavender and Janet A.M. Fyfe
14. Detection of Bartonella spp. DNA in Clinical Specimens using an Internally Controlled Real-time PCR Assay
Anneke M. C. Bergmans and John W.A. Rossen
15. Direct Identification of Multiple Genital Pathogens in Urine using Multiplex PCR-based Reverse Line Blot Assays
Michelle L. McKechnie, Fanrong Kong, and Gwendolyn L. Gilbert
16. Diagnosis of Clostridium difficile Infection using Real-time PCR
Renate Johanna van den Berg, Dennis Bakker, and Ed J. Kuijper
17. Detection of Pathogenic Leptospira spp. through Real-time PCR (qPCR) Targeting the LipL32 Gene
Robyn Anne Stoddard
18. Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli using Loop-mediated Isothermal Amplification
Wataru Yamazaki
19. PCR Detection of Helicobacter pylori in Clinical Samples
Emiko Rimbara, Masanori Sasatsu, and David Y. Graham
20. Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-time PCR
Andrée F. Maheux, Luc Bissonnette, and Michel G. Bergeron
21. Multiplex Real Time PCR (MRT-PCR) for Diarrheagenic Escherichia coli
Francesca Barletta, Theresa J. Ochoa, and Thomas G. Cleary
