About the Book
Preface...
Table of Contents...
Contributing Authors...
Part I Cytological Methods
1. Fluorescence In Situ Hybridization in Oat
Eva Wegel
2. Oat Doubled Haploids Following Maize Pollination
Philip A. Davies and Parminder K. Sidhu
3. Wide Hybridization between Oat and Pearl Millet
Takayoshi Ishii
4. Protocol for Producing Synthetic Polyploid Oats
Toshinobu Morikawa
Part II In Vitro Cultures and Genetic Transformation
5. Manipulation of Oat Protoplasts for Transient Expression Assays
Robyn Roberts, Jincan Zhang, Nicole Mihelish, Danielle Savino, Aurélie M. Rakotondrafara
6. Oat Anther Culture and Use of DH-Lines for Genetic Mapping
Elina Kiviharju, Sirpa Moisander, and Pirjo Tanhuanpää
7. Agrobacterium-Mediated Transformation of Leaf Base Segments
Sebastian Gasparis
Part III Quality Assessment
8. Chromatographic Methods to Evaluate Nutritional Quality in Oat
Gracia Montilla-Bascón, Corey D. Broeckling, Owen A. Hoekenga, Elena Prats, Mark Sorrells, and Julia Isidro-Sánchez
9. Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry
Veronica M.T. Lattanzio and Michelangelo Pascale
10. Multiplex Dipstick Immunoassay for Semi-Quantitative Determination of Fusarium Mycotoxins in Oat
Veronica M.T. Lattanzio and Noan Nivarlet
11. Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
Susanna Oswald, Richard Dietrich, Erwin Märtlbauer, Reinhard Niessner, and Dietmar Knopp
Part IV Genomic Studies
12. M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm
Agnieszka Onyśk and Maja Boczkowska
13. Genotyping-By-Sequencing and its Application to Oat Genomic Research
Yong-Bi Fu and Mo-Hua Yang
14. Genome-Wide Association Analysis using R
Julio Isidro Sánchez, Deniz Akdemir, and Gracia Montilla-Bascón
15. de novo Transcriptome Assembly in Polyploid Species
Juan J. Gutierrez-Gonzalez and David F. Garvin
Part V Proteomics Methods
16. Isolation of Oat (Avena sativa L.) Total Proteins and their Prolamin Fractions for 2-D Electrophoresis
Dorota Nalęcz, Marta Dziuba, and Iwona Szerszunowicz
17. 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and their Prolamin Fractions
Dorota Nalęcz, Iwona Szerszunowicz, Marta Dziuba, and Piotr Minkiewicz
18. Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques used in the Strategy for the Identification of Oat Proteins after 2-DE
Iwona Szerszunowicz, Dorota Nalęcz, and Marta Dziuba
