1. Neurotrophic Factors: An Overview
Stephen D. Skaper
2. Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions
Paola Tirassa, Pamela Rosso, and Angela Iannitelli
3. Culture of Rodent Cortical, Hippocampal, and Striatal Neurons
Laura Facci and Stephen D. Skaper
4. Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum
Stephen D. Skaper and Laura Facci
5. Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents
Stephen D. Skaper and Laura Facci
6. Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays
Christine A. Webber and Douglas W. Zochodne
7. Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening
Aviva M. Tolkovsky and Jack Brelstaff
8. Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents
Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, and Laura Facci
9. Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice
Paola Brun and Hamid I. Akbarali
10. Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation
Laura Facci, Massimo Barbierato, and Stephen D. Skaper
11. Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity
Cinzia Volonté, Adele De Ninno, and Susanna Amadio
12. Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair
Massimo Barbierato, Stephen D. Skaper, and Laura Facci
13. Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia
Laura Facci and Stephen D. Skaper
14. Diolistic Labeling and Analysis of Dendritic Spines
M. Foster Olive, Armani P. Del Franco, and Cassandra D. Gipson
15. Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses
Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi
16. Methods for Quantitative Analysis of Axonal Cargo Transport
Matias Alloatti, Luciana Bruno, and Tomas L. Falzone
17. Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis
Nicholas Radio and Steven Frank
18. Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Hacham, Arnon Fluksman, Ofra Benny, Cezary Marcinkiewicz, and Peter I. Lelkes
19. Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, and Peter I. Lelkes
20. Rat Pancreatic Beta-Cell Culture
Myrian Velasco, Carlos Larqué, Carlos Manlio Díaz-García, Carmen Sanchez-Soto, and Marcia Hiriart
21. Growth and Neurotrophic Factors in Embryonic Stem Cells
Micaela Pannella, Luciana Giardino, Laura Calzà, and Mercedes
