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Neurodegeneration: Methods and Protocols

Neurodegeneration: Methods and Protocols

          
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About the Book

Part I. Neurodegeneration

1. Introduction to Neurodegenerative Diseases and Related Techniques

Hibiki Kawamata and Giovanni Manfredi

2. In vivo and In vitro Determination of Cell Death Markers in Neurons

Nicole Naniche, Daniela Sua, and Piera Pasinelli

3. Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods

Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, and Joseph P. Pierce

Part II. Neural Cell Culture Techniques

4. Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex

Hyun-Jeong Kim and Jordi Magrané

5. Isolation and Culture of Postnatal Spinal Motoneurons

Carol Milligan and David Gifondorwa

6. Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration

Stuart M. Chambers, Yvonne Mica, Lorenz Studer, and Mark J. Tomishima

7. Neural Differentiation of Induced Pluripotent Stem Cells

Mark Denham and Mirella Dottori

Part III. Models of Neurodegeneration

8. Developing Yeast Models of Human Neurodegenerative Disorders

Alejandro Ocampo and Antoni Barrientos

9. Modeling Dopamine Neuron Degeneration in C. elegans

Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, and Kim A. Caldwell

10. Role of the Proteasome in Fly Models of Neurodegeneration

Chun-Hung Yeh, Marlon Jansen, and Thomas Schmidt-Glenewinkel

11. Modeling Neurodegenerative Diseases in Zebrafish Embryos

Angela Laird and Wim Robberecht

12. Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases

Kindiya Geghman and Chenjian Li

13. Modeling Focal Cerebral Ischemia In vivo

Katherine Jackman, Alexander Kunz, and Costantino Iadecola

14. Pharmacological Models of Parkison's Disease in Rodents

Peter Klivenyi and Laszlo Vecsei

15. Behavioral Phenotyping of Mouse Models of Neurodegeneration

Magali Dumont

Part IV. Neurodegenerative Disease Mechanisms - Proteinopathies

16. Characterization of Amyloid Deposits in Neurodegenerative Diseases

Ruben Vidal and Bernardino Ghetti

17. Detection of Protein Aggregates in Neurodegenerative Diseases

Han-xiang Deng, Eileen H. Bigio, and Teepu Siddique

18. Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures

Natura Myeku, Maria Jose Metcalfe, Qian Huang, and Maria Figueiredo-Pereira

Part V. Neurodegenerative Disease Mechanisms - Mitochondria

19. Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium

Andrey Y. Abramov and Michael R. Duchen

20. Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue

Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, and Anatoly A. Starkov

21. Monitoring Mitophagy in Neuronal Cell Cultures

Jianhui Zhu, Ruben K. Dagda, and Charleen T. Chu

Part VI. Neurodegenerative Disease Mechanisms - Biological functions

22. Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging

Davide Tampellini and Gunnar K. Gouras

23. Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices

Doo Yeon Kim and Dora M. Kovacs

24. Imaging Pr


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Product Details
  • ISBN-13: 9781493962204
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Softcover reprint of the original 1st ed. 2011
  • Language: English
  • Returnable: Y
  • Spine Width: 27 mm
  • Weight: 915 gr
  • ISBN-10: 1493962205
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 512
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm


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