Part I: Physiology

1. Phenotyping Jasmonate Regulation of Senescence

Martin A. Seltmann and Susanne Berger

2. Characterizing Jasmonate Regulation of Male Fertility in Arabidopsis

Bryan Thines, Ajin Mandaokar, and John Browse

3. Phenotyping Jasmonate Regulation of Root Growth

Fabian Kellermeier and Anna Amtmann

Part II: Environment

4. Bioassays for Assessing Jasmonate-Dependent Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial Rhizobacteria

Saskia C.M. Van Wees, Johan A. Van Pelt, Peter A.H.M. Bakker, and Corné M.J. Pieterse

5. Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory

Marco Herde, Abraham J.K. Koo, and Gregg A. Howe

6. Pseudomonas syringae Infection Assays in Arabidopsis

Jian Yao, John Withers, and Sheng Yang He

7. Jasmonate Signaling in the Field, Part I: Elicited Changes in Jasmonate Pools of Transgenic Nicotiana attenuata Populations

Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin

8. Jasmonate Signaling in the Field, Part II: Insect-Guided Characterization of Genetic Variations in Jasmonate-Dependent Defenses of Transgenic and Natural Nicotiana attenuata Populations

Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin

Part III: Molecules

9. Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves

Yusuke Jikumaru, Mitsunori Seo, Hideyuki Matsuura, and Yuji Kamiya

10. A Non-Targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding

Gaëtan Glauser and Jean-Luc Wolfender

11. Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach

Bettina Hause, Kati Mielke, and Susanne Forner

12. Jasmonic Acid-Amino Acid Conjugation Enzyme Assays

Martha L. Rowe and Paul E. Staswick

13. Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins

Sandra Fonseca and Roberto Solano

14. Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins

Amparo Cuéllar Pérez, Laurens Pauwels, Rebecca de Clercq, and Alain Goossens

15. Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins

Tiancong Qi, Susheng Song, and Daoxin Xie

16. Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of Regulatory Proteins Involved in Jasmonate Signaling

Volkan Çevik and Kemal Kazan

17. Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA

Johan Memelink

18. Transient Expression Assays in Tobacco Protoplasts

Robin Vanden Bossche, Brecht Demedts, Rudy Vanderhaeghen, and Alain Goossens

19. Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters

Nobukazu Shitan, Akifumi Sugiyama, and Kazufumi Yazaki

20. Expression Analysis of Jasmonate-Responsive Lectins in Plants

Nausicaä Lannoo and Els J.M. Van Damme

Part IV: Omics

21. Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics

Hye Kyong Kim, Young Hae Choi, and Robert Verpoorte

22. Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry