Part I. Single Molecule Techniques

1. Immobilization of Proteins for Single Molecule Fluorescence Resonance Energy Transfer Measurements of Conformation and Dynamics

Ucheor B. Choi, Keith R. Weninger, and Mark E. Bowen

2. Application of Confocal Single Molecule FRET to Intrinsically Disordered Proteins

Benjamin Schuler, Sonja Müller-Späth, Andrea Sorranno, Daniel Nettles

3. Single-Molecule Force Spectroscopy of Chimeric Polyprotein Constructs Containing Intrinsically Disordered Domains

Marco Brucale, Isabella Tessari, Luigi Bubacco, Bruno Samorì

4. Visualization of Mobility by Atomic Force Microscopy

Toshio Ando and Noriyuki Kodera

5. Unequivocal Single-Molecule Force Spectroscopy of Intrinsically Disordered Proteins

Javier Oroz, Rubén Hervás, Alejandro Valbuena & Mariano Carrión-Vázquez

Part II. Methods to Assess Protein Size and Shape

6. Sedimentation Velocity Analytical Ultracentrifugation for Intrinsically Disordered Proteins

Andrés G. Salvay, Guillaume Communie, and Christine Ebel

7. Analysis of Intrinsically Disordered Proteins by Small-Angle X-ray Scattering

Pau Bernadó and Dmitri I. Svergun

8. Small Angle Neutron Scattering (SANS) for the Structural Study of Intrinsically Disordered Proteins in Solution: a Practical Guide

Frank Gabel

9. Dynamic and Static Light Scattering of Intrinsically Disordered Proteins

Klaus Gast and Christian Fiedler

10. Estimation of Intrinsically Disordered Protein Shape and Time-averaged Apparent Hydration in Native Conditions by a Combination of Hydrodynamic Methods

Johanna C. Karst, Ana Cristina Sotomayor-Pérez, Daniel Ladant and Alexandre Chenal

11. Size-Exclusion Chromatography in Structural Analysis of Intrinsically Disordered Proteins

Vladimir N. Uversky

Part III. Methods to Analyze Conformational Behavior

12. Denaturant-induced Conformational Transitions in Intrinsically Disordered Proteins

Paolo Neyroz, Stefano Ciurli, and Vladimir N. Uversky

13. Identification of Intrinsically Disordered Proteins by a Special 2D Electrophoresis

Agnes Tantos, Peter Tompa

14. pH-induced Changes in Intrinsically-disordered Proteins

Matthew D. Smith and Masoud Jelokhani-Niaraki

15. Temperature Induced Transitions in Disordered Proteins Probed by NMR Spectroscopy

Magnus Kjaergaard, Flemming M. Poulsen and Birthe B. Kragelund

16. Analyzing Temperature Induced Transitions in Disordered Proteins by NMR Spectroscopy and Secondary Chemical Shift Analyses

Magnus Kjaergaard, Flemming M. Poulsen & Birthe B. Kragelund

17. Osmolyte-, Binding-, and Temperature-Induced Transitions of Intrinsically Disordered Proteins

Allan Chris M. Ferreon and Ashok A. Deniz

18. Laser Temperature-jump Spectroscopy of Intrinsically Disordered Proteins

Stephen J. Hagen

19. Differential Scanning Microcalorimetry of Intrinsically Disordered Proteins

Sergei E. Permyakov

20. Identifying Disordered Regions in Proteins by Limited Proteolysis

Angelo Fontana, Patrizia Polverino de Laureto, Barbara Spolaore and Erica Frare

21. The Effect of Counter Ions on the Conformation of Intrinsically Disordered Proteins Studied by Size-exclusion Chromatography

Magdalena Wojtas, Tomasz M. Kaplon, Piotr Dobryszycki, Andrzej Ożyhar

22. Mean Net Charge of Intrinsically Disordered Proteins: Experimental Determination of Protein Valence by Electrophoretic Mobility Measurements

Ana Cristina Sotomayor-Pérez, Johanna C. Karst, Daniel Ladant and Alexandre Chenal

23. Protein Characterization by Partitioning in Aqueous Two-Phase Systems

Larissa Mikheeva, Pedro Madeira, and Boris Zaslavsky

Part IV. Mass-Spectrometry

24. Detection and Characterization of Large-scale Protein Conformational Transitions in Solution using Charge