Part I: Analysis of mRNA Translation Regulation
1. An Overview of Methods for Detecting eIF2α Phosphorylation and the Integrated Stress Response
Agnieszka Krzyzosiak, Aleksandra Pitera, and Anne Bertolotti
2. CRISPR-Based Screening for Stress Response Factors in Mammalian Cells
Xiaoyan Guo and Martin Kampmann
3. Multiplexed Analysis of Human uORF Regulatory Functions during the ISR Using PoLib-Seq
Gemma E. May and C. Joel McManus
4. Measuring Bulk Translation Activity in Single Mammalian Cells during the Integrated Stress Response
Alyssa M. English and Stephanie L. Moon
5. Quantitative Translation Proteomics Using mePROD
Kevin Klann and Christian Münch
6. Quantifying the Binding of Fluorescently Labelled Guanine Nucleotides and Initiator tRNA to Eukaryotic Translation Initiation Factor 2
Martin D. Jennings and Graham D. Pavitt
7. Mammalian In Vitro Translation Systems
Yulia Gonskikh, Valentina Pecoraro, and Norbert Polacek
8. Measuring Repeat-Associated Non-AUG (RAN) Translation
Shaopeng Wang and Shuying Sun
9. Analysis of Ribosome Profiling Data
Carine Legrand, Khanh Dao Duc, and Francesca Tuorto
10. Analysis of Translational Control in the Integrated Stress Response by Polysome Profiling
Michael J. Holmes, Jagannath Misra, and Ronald C. Wek
11. High-Resolution Ribosome Profiling for Determining Ribosome Functional States during Translation Elongation
Mohammad Shafieinouri, Britnie Santiago Membreno, and Colin Chih-Chien Wu
12. Fluorescence Intensity-Based eIF2B's Guanine Nucleotide-Exchange Factor Activity Assay
Yusuke Sekine, David Ron, and Alisa F. Zyryanova
Part II: Analysis of Interaction Networks and RNP Granules
13. Collective Learnings of Studies of Stress Granule Assembly and Composition
Hadjara Sidibé and Christine Vande Velde
14. Detecting Stress Granules in Drosophila Neurons
Fabienne De Graeve, Nadia Formicola, Kavya Vinayan Pushpalatha, Akira Nakamura, Eric Debreuve, Xavier Descombes, and Florence Besse
15. Monitoring and Quantification of the Dynamics of Stress Granule Components in Living Cells by Fluorescence Decay after Photoactivation
Anna-Carina Söhnel, Nataliya I. Trushina, and Roland Brandt
16. Probing Protein Solubility Patterns with Proteomics for Insight into Network Dynamics
Xiaojing Sui,
