1. Introduction to Cell Culture
Christina Philippeos, Robin D. Hughes, Anil Dhawan, and Ragai R. Mitry
2. Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes
Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
3. Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells
Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, and Claus-Michael Lehr
4. Culture of Parathyroid Cells
Peyman Björklund and Per Hellman
5. Functional Analysis of Human Islets of Langerhans Maintained in Culture
Shanta J. Persaud, Bo Liu, and Peter M. Jones
6. Conversion of Non-Endocrine Human Pancreatic Cells to Insulin Producing Cells for Treatment of Diabetes
Min Zhao and Guocai Huang
7. Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
María José Gómez-Lechón, Agustín Lahoz, José V.Castell, and María Teresa Donato
8. Human Hepatocytes: Isolation, Culture, and Quality Procedures
Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Büchler, Michael Schoenberg, Jörg Kleeff, Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, Jan Hengstler, and Andreas K. Nussler
9. Preclinical Testing of Virotherapeutics for Primary and Secondary Tumors of the Liver
Martina Zimmermann, Timo Weiland, Michael Bitzer, and Ulrich M. Lauer
10. Isolation and Functional Studies of Human Fetal Gastric Epithelium in Primary Culture
Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard
11. Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells
Jean-François Beaulieu and Daniel Ménard
12. Primary Culture of Human Renal Proximal Tubule Epithelial Cells and Interstitial Fibroblasts
Claire C. Sharpe and Mark E.C. Dockrell
13. Glomerular Epithelial and Mesangial Cell Culture and Characterization
Heather M. Wilson and Keith N. Stewart
14. Isolation and Culture of Human Adipocytes and Adipose Tissue
Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried
15. Primary Culture of Human Adipocyte Precursor Cells: Expansion and Differentiation
Thomas Skurk and Hans Hauner
16. Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroidogenesis
Jerzy F. Galas
17. Human Vascular Smooth Muscle Cell Culture
Diane Proudfoot and Catherine Shanahan
18. Culture of Human Endothelial Cells from Umbilical Veins
Richard C.M. Siow
19. Human Peripheral Blood Mononuclear Cell Culture for Flowcytometric Analysis of Phosphorylated Mitogen-Activated Protein Kinases
Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, and Dimitrios P. Bogdanos
20. Human CD4+CD25highCD127low/neg Regulatory T Cells
Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, and Yun Ma
21. Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Darren A. Plumb, and Mary B. Goldring
22. Isolation and Culture of Human Osteoblasts
Aliso
