About the Book
1. Long-Term Expansion of Primary Hepatocyte Organoids
Thomas A. Kluiver, Lianne J. Kraaier, and Weng Chuan Peng
2. Generation of in vivo traceable hepatocyte-like cells from human iPSCs
Candice Ashmore-Harris and Gilbert O. Fruhwirth
3. Generation of hepatocyte organoids from human iPS cells
Giuseppe Pettinato
4. Induction of bile canaliculi forming hepatocytes from human induced pluripotent stem cells
Lavinija Matakovic, Arend W. Overeem, Karin Klappe, and Sven C. D. van IJzendoorn
5. Human hepatocyte transduction with adeno-associated virus vector
Zhenwei Song, Wenwei Shao, Liujiang Song1, Xieolei Pei, and Chengwen Li
6. Hepatocyte-directed delivery of Lipid-encapsulated small interfering RNA
Laura Morán, Marius Maximilian Woitok, Matthias Bartneck, and Francisco Javier Cubero
7. Co-culture model for hepatitis B virus infection using iPSC-derived liver progenitor cells and liver sinusoidal endothelial cells
Misao Himeno, Shin-Wei Chen, and Taketomo Kido
8. In vitro assay system to detect drug-induced bile acid-dependent cytotoxicity using hepatocytes
Akinori Takemura, and Kosei Itoh
9. Evaluation of urea cycle activity by metabolic flux analysis using mass spectrometry
Yasuharu Ueno, Takuji Maeda, Satoshi Okamoto, and Hideki Taniguchi
10. Live Cell Imaging of Biosynthetic Protein Transport in Hepatocytes
Francisco Lázaro-Diéguez and Anne Müsch
11. Collagen sandwich culture of primary hepatocytes for image-based investigations
Dong Fu
12. Assessment of Hepatocyte Ploidy by Flow Cytometry
Yinhua Jin, Teni Anbarchian, and Roel Nusse
13. Isolation of small hepatocyte-like progenitor cells from retrorsine/partial hepatectomy rat livers by laser micro-dissection
Norihisa Ichinohe, Naoki Tanimizu, and Toshihiro Mitaka
14. Application of EdU-based DNA synthesis assay to measure hepatocyte proliferation in situ during liver regeneration
Amit Ghosh, Awais Ullah Ihsan, Madhuparna Nandi, Maryse Cloutier, Md Gulam Musawwir Khan, Sheela Ramanathan and Subburaj Ilangumaran
15. Quantification of clonal expansion of hepatocytes in normal and injured liver
Feng Chen
16. Evaluation of SOX9-positive hepatocytes in human liver specimens and mature mouse hepatocytes
Yuji Suzuki, Tokio Sasaki, Keisuke Kakisaka, Hiroaki Abe, and Yasuhiro Takikawa
17. Identification of genes regulating hepatocyte injury by a genome-wide CRISPR-Cas9 Screen
Katherine Shortt and Daniel P. Heruth
18. Identification of subtypes of HCC using bioinformatics and the hepatocyte differentiation model
Wei Cheng, Yan Zhang, and Ming Liu
19. 3D culture of primary patient-derived hepatoblastoma tumoroids
Peng V. Wu and Roel Nusse
20. Preparation of functional heaptocytes
Ayumu Okumura and Naoki Tanimizu