Part I: Glyco-Engineering of Therapeutic Proteins

1. Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells

Linda Lindberg, Jining Liu, and Jan Holgersson

2. Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells

Nassimal El Maï, Sandrine Donadio-Andréi, Chloé Iss, Valérie Calabro, and Catherine Ronin

3. Glycoengineered Pichia-Based Expression of Monoclonal Antibodies

Dongxing Zha

4. N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae

Christelle Arico, Christine Bonnet, and Christophe Javaud

5. Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells

Sylvie Juliant, Marylêne Lévêque, Pierre Cérutti, Annick Ozil, Sylvie Choblet, Marie-Luce Violet, Marie-Christine Slomianny, Anne Harduin-Lepers, and Martine Cérutti

Part II: Glycoanalytics

6. Nano LC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody

Elsa Wagner-Rousset, Christine Schaeffer-Reiss, Audrey Bednarczyk, Nathalie Corvaïa, Alain Van Dorsselaer, and Alain Beck

7. Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry

Marie-Claire Janin-Bussat, Laure Tonini, Céline Huillet, Olivier Colas, Christine Klinguer-Hamour, Nathalie Corvaïa, and Alain Beck

8. Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support

Arnaud Delobel, Fabrice Cantais, Anicet Catrain, Erell Dereux, and Géry Van Vyncht

9. Mass Spectrometric Analysis of O-Linked Oligosaccharides from Various Recombinant Expression Systems

Diarmuid T. Kenny, Stefan Gaunit, Catherine A. Hayes, Anki Gustafsson, Magnus Sjöblom, Jan Holgersson, and Niclas G. Karlsson

10. Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC

T. Shantha Raju

11. Application of Capillary Electrophoresis in Glycoprotein Analysis

Richard R. Rustandi, Carrie Anderson, and Melissa Hamm

12. Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology

Grace Chen, Sha Ha, and Richard R. Rustandi

13. Hydrophobic Interaction Chromatography to Analyze Glycoproteins

Richard R. Rustandi

14. Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7

Ludovic Landemarre and Eric Duverger

15. Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation

W. Blaine Stine, Jr.

Part III: Glycoprotein Complexes Characterization

16. Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes

Cédric Atmanene, Elsa Wagner-Rousset, Nathalie Corvaïa, Alain Van Dorsselaer, Alain Beck, and Sarah Sanglier-Cianferani

17. Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry

Damian Houde and John R. Engen

18. Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study

Victor H. Obungu, Valentina Gelfanova, and Lihua Huang

Part IV: PK/PD Assays for Therapeutic Antibodies

19. Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Des