1. Real-Time PCR and Multiplex Approaches
Olga L. Gurvich and Mikhail Skoblov
2. Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs
Sweta Rani and Lorraine O'Driscoll
3. Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals
Jai Prakash Mehta
4. Software and Tools for Microarray Data Analysis
Jai Prakash Mehta and Sweta Rani
5. Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells
Anne M. Friel, John Crown, and Lorraine O'Driscoll
6. Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay
Craig S. April and Jian-Bing Fan
7. MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs
Erica Hennessy and Lorraine O'Driscoll
8. Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases
Serena Germano and Lorraine O'Driscoll
9. 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins
Paula Meleady
10. Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis
Kathy Gately, Keith Kerr, and Ken O'Byrne
11. Immunohistochemical and Immunofluorescence Procedures for Protein Analysis
Kishore Reddy Katikireddy and Finbarr O'Sullivan
12. Advanced Microscopy: Laser Scanning Confocal Microscopy
Orla Hanrahan, James Harris, and Chris Egan
13. Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling
Sweta Rani, Keith O'Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek Radomski, John Crown, and Lorraine O'Driscoll
14. Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties
Stephen T. Buckley, Anthony M. Davies, and Carsten Ehrhardt
