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Estudios Estructurales de Lectinas de Algas y de Vegetales superiores

Estudios Estructurales de Lectinas de Algas y de Vegetales superiores

          
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About the Book

El objetivo global del trabajo fue profundizar en aspectos estructurales y evolutivos de lectinas vegetales. Por una parte, se ha caracterizado las lectinas de algas marinas rojas Hypnea cervicornis e Hypnea musciformis. Las lectinas contienen 90 aminoácidos, presentan dos dominios homologos entre sí dispuestos en tandem y no muestran similitud con ninguna proteína descrita, indicando que son miembros fundadores de una nueva familia de lectinas. Por otra parte, se centró en la determinación de las bases estructurales del equilibrio dímero tetrámero dependiente del pH que presentan algunas lectinas de la tribu Diocleinae de la familia Papilionoideae de las leguminosas. Para ello, se ha empleado un abordaje basado en técnicas de Biología Molecular, Biofísica y Biología Estructural. Las proteínas recombinantes de Dioclea guianensis y Dioclea grandiflora son bioquimicamente indistinguibles de sus homólogas aisladas de semillas. Se ha logrado generar el equilíbrio dímero-tetrámero dependiente del pH a partir de la lectina r-DGL, tetramérica en el intervalo de pH de 4-8.5. La existencia del equilibrio dímero-tetrámero se correlaciona con una conformación retraída de los bucles 117-122.


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Product Details
  • ISBN-13: 9783844339475
  • Publisher: Editorial Academica Espanola
  • Binding: Paperback
  • Language: Spanish
  • Returnable: N
  • Spine Width: 8 mm
  • Width: 152 mm
  • ISBN-10: 3844339477
  • Publisher Date: 15 Jun 2011
  • Height: 229 mm
  • No of Pages: 140
  • Series Title: Spanish
  • Weight: 217 gr


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