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Efecto genotóxico y mutagénico de la desnutrición moderada y grave en reticulocitos y eritrocitos de rata

Efecto genotóxico y mutagénico de la desnutrición moderada y grave en reticulocitos y eritrocitos de rata

          
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About the Book

Tesis Doctoral / Disertación del año 2016 en eltema Biología - Genética / Ingeniería genética, Idioma: Español, Resumen: La DN es un padecimiento que daña las funciones celulares de manera progresiva en el organismo. Es la causa del 45 % de las muertes en los niños menores de 5 años de edad, y aunque se han estudiado ampliamente sus efectos a nivel citogenético, a nivel génico no se cuenta con suficientes reportes que muestren si es posible relacionar el grado de DN con la susceptibilidad génica. En el manejo clínico de la DN, los antibióticos forman parte de la mayoría de los protocolos, no obstante, existe poco análisis de los efectos del actual método de tratamiento en los que se emplean diferentes antibióticos de rutina como la TMPSMX. En este trabajo se evaluaron los efectos genotóxico y mutagénico del DN2° y del DN3° con la combinación de los ensayos de MN y Pig-a para determinar el daño al ADN en RET y E de ratas BN y con DN2° ó DN3° expuestas y no expuestas a ENU ó a TMP-SMX. Ratas de la cepa Wistar fueron evaluadas por 63 días después de la exposición a ENU y 45 días después de ser tratadas con TMP-SMX donde se monitorearon los porcentajes de MN y las frecuencias de mutantes Piga. Los resultados muestran que la DN2° y la DN3° se relacionan con un efecto genotóxico y mutagénico al incrementar el porcentaje de MN y la frecuencia de mutantes Pig-a. Los animales expuestos al ENU presentaron incrementos dosis y tiempo dependientes. Al comparar los efectos inducidos entre estos grupos se observó que no hubo diferencias significativas entre los animales BN y con DN2°. Por otra parte, se encontró un efecto mutagénico persistente con la dosis de 100/500 mg TPM-SMX y DN3°. El DN3° tiene mayor efecto genotóxico en comparación con el efecto producido por la DN2°. No se observaron diferencias en el efecto mutagénico relacionado con el grado de DN. De acuerdo a los resultados obtenidos, es necesario continuar con estudios complementarios en ambos grados de DN que fo


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Product Details
  • ISBN-13: 9783668434943
  • Publisher: Grin Publishing
  • Binding: Paperback
  • Language: Spanish
  • Returnable: N
  • Weight: 168 gr
  • ISBN-10: 3668434948
  • Publisher Date: 24 May 2017
  • Height: 210 mm
  • No of Pages: 120
  • Spine Width: 7 mm
  • Width: 148 mm


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