Part I: Introduction
1. Marburg and Ebola Viruses: A Look Back and Lessons for the Future
Hans Dieter Klenk and Werner Slenczka
2. 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies
Allison Groseth and Thomas Hoenen
3. Ebolavirus: An Overview of Molecular and Clinical Pathogenesis
Veronica Vine, Dana P. Scott, and Heinz Feldmann
Part II: Studying Ebolaviruses Under Biosafety Level 2 Conditions
4. Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms
Rachel B. Brouillette and Wendy Maury
5. Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins
Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, and Wendy Maury
6. Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems
Tessa Cressey, Kristina Brauburger, and Elke Mühlberger
7. Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation
Logan Banadyga and Hideki Ebihara
8. Modeling Ebolavirus Budding with Virus-Like Particles
Olivier Reynard and Mathieu Mateo
9. Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays
Nadine Biedenkopf and Thomas Hoenen
10. Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins
Priya Luthra and Christopher Basler
11. Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA Kristina Brauburger, Tessa Cressey, and Elke Mühlberger
Part III: Studying Infectious Ebolaviruses In Vitro
12. A Semi-Automated High Throughput Microtitration Assay for Filoviruses
Claire Marie Filone, David Miller, and Victoria Wahl-Jensen
13. Generation of Recombinant Ebola Viruses Using Reverse Genetics
Allison Groseth
14. Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals
Thomas Hoenen
15. Live-Cell Imaging of Filoviruses
Gordian Schudt, Olga Dolnik, and Stephan Becker
16. Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds
Darryl Falzarano
17. Analysis of the Cellular Stress Response During Ebola Virus Infection by Immun
