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Determinazione della frazione mutata di NPM1 nell'AML mediante dPCR

Determinazione della frazione mutata di NPM1 nell'AML mediante dPCR

          
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About the Book

La mutazione del gene NPM1 (Nucleofosmina) è una delle mutazioni acquisite più frequentemente osservate nella leucemia mieloide acuta (AML). Tra i numerosi tipi di mutanti NPM1, la mutazione di tipo A rappresenta l'80%. Il monitoraggio di questo marcatore è indicato di routine nella gestione della LMA. Attualmente viene monitorato mediante RT-qPCR. I risultati sono espressi come rapporto "mutato/normale" di mutanti NPM1 e richiedono l'uso di un gene di riferimento.La PCR digitale è una tecnologia recente che consente la quantificazione assoluta di un evento genetico raro. I punti di forza della PCR digitale sono numerosi: il gene di riferimento è identico al target di interesse, la sensibilità è praticamente identica a quella della RT-qPCR e non è necessario utilizzare saggi plasmidici. In questo contesto, abbiamo sviluppato un metodo di PCR digitale per il biomarcatore NPM1 di tipo A, determinando i parametri ottimali della tecnica per questo rilevamento. In conclusione, la PCR digitale sembra essere uno strumento importante per il monitoraggio della malattia residua.


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Product Details
  • ISBN-13: 9786206316060
  • Publisher: KS Omniscriptum Publishing
  • Publisher Imprint: Edizioni Sapienza
  • Height: 229 mm
  • No of Pages: 72
  • Spine Width: 4 mm
  • Width: 152 mm
  • ISBN-10: 6206316068
  • Publisher Date: 06 Aug 2023
  • Binding: Paperback
  • Language: Italian
  • Returnable: N
  • Weight: 118 gr


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