Preface

Introduction

Chapter 1. Bases of the derivative spectrophotometry

1.1 The main law of light absorption by a substance

1.1.1 Reasons of deviation from Bouguer's law

1.2 Correctness and accuracy of spectrophotometric data

1.2.1 Main factors influencing on accuracy of spectrophotometric measurements

1.2.2 Difference (differential) spectrophotometry

1.2.3 Measurement errors of the difference spectrophotometry

1.3 Derivative spectrophotometry

1.3.1 Methods of derivative signal registration and diagrams of differential analyzers

1.3.2 Parameter optimization of the differentiating circuit

1.3.3 The derivative spectrophotometry of difference spectra

1.3.4 Method of the pulse amplitude-modulated fluorescence for the solution of ecologicalbiochemical problems

Chapter 2. The derivative spectrophotometry method for analysis of biologically active substances

2.1 The derivative spectrophotometry for analysis of a number of guanidine preparations

2.2 Сhelating ability of 1,3-bis-((p-chlorobenzylidene)amino) guanidine: complexes with Ca²⁺and La³⁺ ions

2.3 The special features of the Ca²⁺binding by mono-, bis- and tris-substituted guanidine derivatives

2.4 Special features of interaction of bis-((chlorobenzylidene)amino)guanidine derivatives with Ca²⁺ depending on the chlorine atom position in the molecule

2.5 The specific character of Ca²⁺ interaction with ((benzylidene)amino)guanidine derivatives containig electron-donor or electron-acceptor substituters

2.6 Special features of calcium ions interaction with bis-((4-hydroxy-3-methoxybenzilidene)amino)guanidine and bis-((4-cyanobenzilidene)amino) guanidine

2.7 The proof of polycomponent composition of the promising antitumor drug "Ukrain"

2.8 Derived spectra application for the analysis of derived forms of nondepolarizing muscle relaxant tercuronium, of vitamins and hormones

2.8.1 Comparative analysis of tercuronium derivatives

2.8.2 The reasonability of derived spectra application for the analysis of commercial preparations of vitamins and hormones

2.9 The importance of the derivative spectrophotometry in modern studies of aromatic amino acids and proteins

2.9.1 Special features of derived spectra of phenylalanine, tyrosine and tryptophan

2.9.2 Special features of phenylalanine spectra change caused by influence of gamma radiation

2.9.3 Specific character of tyrosine spectra changes under influence of gamma-radiation

2.9.4 The character of tryptophan derived spectra change under influence of gamma-irradiation

2.9.5 The comparative characteristic of albumin denaturation spectral changes under thermal and radiation exposures

2.9.6 Changes of the gamma globulin optical spectra under γ - irradiation

2.9.7 Special features of γ-globulin spectra changes during γ-globulin denaturation caused by thermal and radiation exposure

2.9.8 The coupling of albumin derived spectra change with the determination accuracy of albumin / globulin coefficient for radiation injuries

Chapter 3. Applicability of the DSHO method in the work with pigments of plants and animals

3.1 Derived spectra of high orders for some carotenoids

3.2 Neoxanthin as a probable key product of formation of α- and β- carotenoid derivatives

3.3 Metabolic transformations of labeled 14C- or 3H- carotene in animal tissues

3.4 Importance of the derivative spectrophotometry for study of alternative ways of carotenoids biosynthesis in Procaryota and Eucaryota

3.5 Possibility of participation of α-ketoglutaric acid funds in carotenoids biosynthesis in chloroplasts

3.6 Malic acid as the source for carotenoids synthesis in plants with С4-