Part I. Perspectives
1. Limbal Epithelial Cell Therapy: Past, Present and Future
Tor Paaske Utheim
2. The Artificial Cornea
Naresh Polisetti, Mohammad Mirazul Islam, and May Griffith
Part II. Corneal Stem Cell Enrichment and Characterisation
3. Clonal Analysis of Limbal Epithelial Stem Cell Populations
Ursula Schlootzer-Schrehardt
4. The Use of Bromodeoxyuridine Incorporation Assays to Assess Corneal Stem Cell Proliferation
Ashley M. Crane and Sanjoy K. Bhattacharya
5. Enrichment of Human Corneal Epithelial Stem/Progenitor Cells by Magnetic Bead Sorting Using SSEA4 as a Negative Marker
Martin N. Nakatsu and Sophie X. Deng
6. Limbal Epithelial Stem Cell Identification using Immunoblotting Analysis
Bernice Wright and Che J. Connon
Part III. Corneal Cell Culture
7. The Culture of Limbal Epithelial Cells
Tor Paaske Utheim, Torstein Lyberg, and Sten Ræder
8. The Culture of Limbal Stromal Cells and Corneal Endothelial Cells
Naresh Polisetti and Nancy C. Joyce
Part IV. Development of the Artificial Cornea
9. The Formation of a Novel Tissue-Engineered Cornea using Collagen Scaffolds and Limbal Stem Cells
Shengli Mi and Che J. Connon
10. Fabrication of a Human Recombinant Collagen-based Corneal Substitute using Carbodiimide Chemistry
Mohammad Mirazul Islam, May Griffith, and Kimberley Merrett
11. Fabrication of a Corneal-Limbal Tissue Substitute Using Silk Fibroin
Laura J. Bray, Karina A. George, Shuko Suzuki, Traian V. Chirila, and Damien G. Harkin
12. Cultivation of Limbal Epithelial Cells on Electrospun Poly (lactide-co-glycolide) Scaffolds for Delivery to the Cornea
Pallavi Deshpande, Charanya Ramachandran, Virender S. Sangwan, and Sheila MacNeil
13. The Growth and Delivery of Mesenchymal and Limbal Stem Cells using Copolymer Polyamide 6/12 Nanofiber Scaffolds
Vladimir Holan, Eliska Svobodova, and Peter Trosan
14. Encapsulation and Culture of Mammalian Cells including Corneal Cells in Alginate Hydrogels
Nicola C. Hunt and Liam M. Grover
15. In vivo Confocal Microscopy of the Cornea to Assess Tissue Regenerative Response after Biomaterial Implantation in Humans
Neil Lagali, May Griffith, and Per Fagerhorn
