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Contributions from the Zoological Laboratory for the Years 1913-14, Vol. 19: Twenty Separata (Classic Reprint): Twenty Separata (Classic Reprint)

Contributions from the Zoological Laboratory for the Years 1913-14, Vol. 19: Twenty Separata (Classic Reprint): Twenty Separata (Classic Reprint)

          
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About the Book

Excerpt from Contributions From the Zoological Laboratory for the Years 1913-14, Vol. 19: Twenty Separata

I am indebted to The Wistar Institute of Anatomy and Biology for the albino rats used in the study and for the use of its labora tory equipment. I am indebted to Dr. Stotsenburg, of the Insti tute, for a. Supply of rats as needed and for courteous attention; to Dr. H. D. King for a method of imbedding and staining; to Dr. S. Batai for valuable suggestions. To Dr. H. H. Donaldson I am especially indebted for constant aid and criticism.

My method of work was in general as follows. Healthy rats of the following ages were used: 1, 4, 6, 7, 12, 15, 18, 20, 30, 52, 70, about 120 days old, and one about two years old. In all, some twenty-five different animals were studied. Record was made of the weight, body-length and sex. After chloroforming the animal, the brain and cord were quickly removed, fixed in Carnoy's fluid and imbedded, the younger specimens in paraffin the older in celloidin and paraffin. Frontal sections were then cut at 8 micra and stained with either iron-alum-haematoxylin or thionin, fol lowed by eosin or erythrosin. Thionin was found to differentiate the mitotic figures with sufficient clearness for this study. By using the mechanical stage, the sections were thoroughly explored with a magnification sufficient to detect mitoses. Only those cells which showed mitotic figures clearly were enumerated; the earlier stages of prophase, being more difficult to identify, were not considered. The Zeiss 4 mm. Objective and N o. 4 eye piece usually sufficed; in doubtful cases the 2 mm. Oil immersion Objective and eye-pieces higher than N o. 4 were employed.

Two methods of record were used. With the younger material, diagrams representing the sections studied were outlined and on these the approximate location of the dividing cells was indicated, the number 1 being employed for the cells in section N o. 1, the number 2 for those in section No. 2, etc. (figs. 1 to When the examination of a series was complete, this method showed graphically the distribution of the dividing cells in that portion of tissue both in cross section and longitudinally. The other method, usually employed for the older material, was to tally the cells as discovered opposite the number of the section, regional location being indicated by columns if desired.

About the Publisher

Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com

This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.


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Product Details
  • ISBN-13: 9781527917774
  • Publisher: Forgotten Books
  • Publisher Imprint: Forgotten Books
  • Language: English
  • ISBN-10: 1527917770
  • Publisher Date: 15 Feb 2019
  • Binding: Paperback


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