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Comercialização de quitosano obtido a partir de resíduos marinhos

Comercialização de quitosano obtido a partir de resíduos marinhos

          
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About the Book

Este estudo teve como principal objetivo detetar a presença de bactérias produtoras de quitinase em resíduos marinhos como os caranguejos Brachyura. A caraterização dos isolados bacterianos foi efectuada através de métodos bioquímicos e de identificação molecular. Na caraterização morfológica dos isolados bacterianos, foram efectuados testes de coloração de Gram e de motilidade. A identificação posterior foi efectuada por métodos bioquímicos. Para a identificação de isolados bacterianos seleccionados por sequenciação parcial do ARN 16s r, o ADN foi isolado pelo método convencional. Seguiu-se a reação em cadeia da polimerase (PCR) para amplificar o ADN. A separação posterior do tamanho foi efectuada por eletroforese em gel de agarose. Identificação molecular dos isolados bacterianos através da sequência de ARNr 16S. O ADN isolado das culturas puras foi amplificado. O produto de PCR 16S rRNA amplificado foi sequenciado. A pesquisa de semelhança de sequências para a sequência de 16S rRNA foi efectuada através de BLAST. Os organismos foram identificados como Bacillus albus e Bacillus cereus.


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Product Details
  • ISBN-13: 9786207904914
  • Publisher: KS Omniscriptum Publishing
  • Publisher Imprint: Edicoes Nosso Conhecimento
  • Height: 229 mm
  • No of Pages: 76
  • Spine Width: 5 mm
  • Width: 152 mm
  • ISBN-10: 6207904915
  • Publisher Date: 31 Jul 2024
  • Binding: Paperback
  • Language: Portuguese
  • Returnable: N
  • Weight: 123 gr


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Comercialização de quitosano obtido a partir de resíduos marinhos
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