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Clonación y caracterización del antígeno celular porcino CD47

Clonación y caracterización del antígeno celular porcino CD47

          
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About the Book

Esta Tesis se presenta la identificación y clonación del ADNc que codifica para el homólogo porcino de la proteína asociada a integrina (IAP o CD47), la localización cromosómica del gen correspondiente y la caracterización funcional de dicha molécula. Se generó una sonda molecular específica y el rastreo de una genoteca de ADNc de músculo liso de aorta de cerdo identificó siete clones conteniendo todos ellos secuencias idénticas. Los clones comprendían una fase abierta de lectura que codificaba un péptido señal putativo de 18 aminoácidos, una secuencia de 122 aminoácidos correspondiente a un dominio extracelular de tipo inmunoglobulina variable (IgV) seguida por una región de 147 aminoácidos conteniendo cinco dominios que atraviesan la membrana y una cola citoplásmica de 16 aminoácidos. El CD47 porcino se expresaba de modo estable en las células CHO transfectadas y se unía a Trombospondina humana (TSP). El anticuerpo BRIC-126, anti-CD47 humano, inhibía la unión de plaquetas y de células transfectadas con CD47 a TSP humana y a fibrinógeno porcino, en tanto que no tenía efecto alguno sobre las células CHO control.CD47 se localiza en la región q42-(1/2q46) del cromosoma 13.


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Product Details
  • ISBN-13: 9783847353423
  • Publisher: Eae Editorial Academia Espanola
  • Publisher Imprint: Eae Editorial Academia Espanola
  • Height: 229 mm
  • No of Pages: 180
  • Series Title: Spanish
  • Weight: 272 gr
  • ISBN-10: 384735342X
  • Publisher Date: 15 Mar 2012
  • Binding: Paperback
  • Language: Spanish
  • Returnable: N
  • Spine Width: 10 mm
  • Width: 152 mm


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