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Cell Separation: Fundamentals, Analytical and Preparative Methods

Cell Separation: Fundamentals, Analytical and Preparative Methods

          
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About the Book

Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various biological applications has increased. Ef?cient and cost-effective techniques for fr- tionation and isolation of target cell types are necessary to provide pure cell populations for diagnostics, biotechnological and biomedical applications. One can see a considerable need, both in biomedical research and in di- nostic medicine, for the speci?c separation of a discrete population of cells from a mixture. For example, in the ?eld of tissue engineering, isolation of stemcellsfromtissuesororgansisofparticularlygreatimportance.Moreover, understanding cell developmental pathways becomes increasingly signi?cant as diagnosis and treatment of diseases turns more to the molecular level. The diagnosis of cell-related diseases requires methods of detection, isolation and theanalysisofindividualcells, regardlessoftheirrelativecontentinthetissue. Since cell-based therapies now turn towards more realistic medical options, developing an effective separation system for large-scale cell separation has becomechallengingresearchgoalforcellbiologistsandbiotechnologists.The ideal technique should provide in a short time a good yield of cells with high puritywhile maintaining cellfunction.Despite the growingneed formethods to separate cells into cell subpopulations, the existing cell-separation te- niques stillhave somelimitations when the desired degree ofperformance on apreparativescaleisrequired.Wewillseemoreresearchfocusinthisdirection in the future. The traditional techniques of micro?ltration, ultra?ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci?cityandproblemswithscalingup


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Product Details
  • ISBN-13: 9783642094545
  • Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
  • Binding: Paperback
  • Height: 156 mm
  • No of Pages: 203
  • Series Title: Advances in Biochemical Engineering & Biotechnology (Hardcover)
  • Sub Title: Fundamentals, Analytical and Preparative Methods
  • Width: 234 mm
  • ISBN-10: 3642094546
  • Publisher Date: 19 Nov 2010
  • Edition: 1st ed. Softcover of orig. ed. 2007
  • Language: English
  • Returnable: Y
  • Spine Width: 12 mm
  • Weight: 313 gr


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