1. Cell Migration in Three Dimensions
Coert Margadant
Part I: The Cell Biology of Cell Migration
2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis
Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, and David A. Calderwood
3. Probing the ER-Focal Adhesion Link during Cell Migration
Noemi Antonella Guadagno and Cinzia Progida
4. Mapping the Localization of Proteins within Filopodia Using FiloMap
Guillaume Jacquemet
5. Extended Methods for 2D Confinement
Juan Manuel García-Arcos, Kevin Gateau, Larisa Venkova, and Matthieu Piel
6. Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63
Bong Hwan Sung and Alissa M. Weaver
7. Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices
Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf
Part II: Developmental Model Systems to Assess Cell Migration during Morphogenesis
8. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila
Maik C. Bischoff and Sven Bogdan
9. Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells
Antonius L. van Boxtel
10. Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure
Matteo A. Molè, Gabriel L. Galea, and Andrew J. Copp
11. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila
Sofia J. Araújo and Marta Llimargas
12. A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland
Edouard Hannezo and Colinda L.G.J. Scheele
13. Analysis of Integrin-Dependent Melanoblast Migration during Development
Amanda Haage and Guy Tanentzapf
Part III: Cell Migration in Cancers and the Tumor Microenvironment
14. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices
David Remy, Anne-Sophie Macé, Philippe Chavrier, and Pedro Monteiro
15. Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion
Jian Zhang and Cynthia A. Reinhart-King
16. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids
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