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Cell Culture Engineering IV: Improvements of Human Health

Cell Culture Engineering IV: Improvements of Human Health

          
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About the Book

Introduction: Applications of Cell Culture for the improvement of human health.- Cultural and physiological factors affecting expression of recombinant proteins.- Selecting and designing cell lines for improved physiological characteristics.- Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor.- Diverse effects of essential (n-6 and n-3) fatty acids on cultured cells.- Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production.- Growth rate suppression of cultured mammalian cells enhances protein productivity.- Growth and interferon-? production in batch culture of CHO cells.- Metabolic and kinetic studies of hybridomas in exponentially fed-batch cultures using T-flasks.- Adaptation of mammalian cells to non-ammoniagenic media.- Effect of endogenous proteins on growth and antibody productivity in hybridoma batch cultures.- Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process.- Low temperature cultivation - A step towards process optimisation.- Induction of apoptosis in oxygen-deprived cultures of hybridoma cells.- Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures.- Recombinant protein expression in a Drosophila cell line: comparison with the baculovirus system.- Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells.- Relationship between oxygen uptake rate and time of infection of Sf9 insect cells infected with a recombinant baculovirus.- Optimization of vaccine production for animal health.- Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells.- The analysis of protein pharmaceuticals: Near future advances.- The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin.- Effect of lipid supplements on the production and glycosylation of recombinant interferon-? expressed in CHO cells.- Role of environmental conditions on the expression levels, glycoform pattern and levels of sialytransferase for hFSH produced by recombinant CHO cells.- Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines.- Interaction of cell culture with downstream purification: a case study.- Evaluation of membranes for use in on-line cell separation during mammalian cell perfusion processes.- Vortex flow filtration of mammalian and insect cells.- Automated immunoanalysis systems for monitoring mammalian cell cultivation processes.- A direct computer control concept for mammalian cell fermentation processes.- Automated monitoring of cell concentration and viability using an image analysis system.- Software sensors for the monitoring of perfusion cultures: Evaluation of the hybridoma density and the medium composition from glucose concentration measurements.- Design of a bubble-swarm bioreactor for animal cell culture.- Cells and bubbles in sparged bioreactors.- Quantitative investigations of cell-bubble interactions using a foam fractionation technique.- Prediction of mechanical damage to animal cells in turbulence.- Repeated-batch cultures of Baby Hamster Kidney cell aggregates in stirred vessels.- Towards the development of a bioartificial pancreas: immunoisolation and NMR monitoring of mouse insulinomas.- Overview of a quality assurance / quality control compliance program consistent with FDA regulations and policies for somatic cell and gene therapies: a four year experience.- Author index.- Keyword index.


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Product Details
  • ISBN-13: 9780792333531
  • Publisher: Kluwer Academic Publishers
  • Publisher Imprint: Kluwer Academic Publishers
  • Edition: Reprinted from CYTOTECHNOLOGY, 15:1-3
  • Language: English
  • Returnable: Y
  • Spine Width: 22 mm
  • Weight: 1006 gr
  • ISBN-10: 0792333535
  • Publisher Date: 31 Jan 1995
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 384
  • Series Title: Current Applications of Cell Culture Engineering
  • Sub Title: Improvements of Human Health
  • Width: 203 mm


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