About the Book
1. Challenges for Assessing the Performance of Biomass Degrading Biocatalysts
Michael E. Himmel, Stephen R. Decker, and David K. Johnson
Part I: Biomass Feedstock and Cellulose
2. Overview of Computer Modeling of Cellulose
Malin Bergenstråhle-Wohlert, and John W. Brady
3. Imaging Cellulose Using Atomic Force Microscopy
Shi-You Ding and Yu-San Liu
4. Preservation and Preparation of Lignocellulosic Biomass Samples
for Multi-scale Microscopy Analysis
Bryon S. Donohoe, Peter N. Ciesielski, and Todd B. Vinzant
5. Coherent Raman Microscopy Analysis of Plant Cell Walls
Yining Zeng, Michael E. Himmel, and Shi-You Ding
6. Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling
Sivakumar Pattathil, Utku Avci, Jeffrey S. Miller, and Michael G. Hahn
7. Immunological Approaches To Plant Cell Wall and Biomass Characterization: Immunolocalization of Glycan Epitopes
Utku Avci, Sivakumar Pattathil, and Michael G. Hahn
8. A Method to Evaluate Biomass Accessibility in Wet State Based on Thermoporometry
Bon-Wook Koo and Sunkyu Park
Part II: Plant Cell Wall
9. Cellulase Processivity
David B. Wilson and Maxim Kostylev
10. A Simple Method for Determining Specificity of Carbohydrate-Binding Modules for Purified and Crude Insoluble Polysaccharide Substrates
Oren Yaniv, Sadanari Jindou, Felix Frolow, Raphael Lamed, and Edward A. Bayer
11. Bacterial Cadherin Domains as Carbohydrate Binding Modules: Determination of Affinity Constants to Insoluble Complex Polysaccharides
Milana Fraiberg, Ilya Borovok, Ronald M. Weiner, Raphael Lamed, and Edward A. Bayer
12. Affinity Electrophoresis as a Method for Determining Substrate-Binding Specificity of Carbohydrate-Active Enzymes for Soluble Polysaccharides
Sarah Moraïs, Raphael Lamed, and Edward A. Bayer
13. Single-Molecule Tracking of Carbohydrate-Binding Modules on Cellulose Using Fluorescence Microscopy
Yu-San Liu, Shi-You Ding, and Michael E. Himmel
14. Bioprospecting Metagenomics for New Glycoside Hydrolases
Jack Gilbert, Luen-Luen Li, Safiyh Taghavi, Sean M. McCorkle, Susannah Tringe, and Daniel van der Lelie
15. Anaerobic High-Throughput Cultivation Method for Isolation
of Thermophiles Using Biomass-Derived Substrates
Scott D. Hamilton-Brehm, Tatiana A. Vishnivetskaya, Steve Allman, Jonathan Mielenz, and James G. Elkins
16. Assessing the Protein Concentration in Commercial Enzyme Preparations
William S. Adney, Nancy Dowe, Edward W. Jennings, Ali Mohagheghi,
John Yarbrough, and James D. McMillan
17. Reducing the Effect of Variable Starch Levels in Biomass Recalcitrance Screening
Stephen R. Decker, Melissa Carlile, Michael J. Selig, Crissa Doeppke, Mark Davis, Robert Sykes, Geoffrey Turner, and Angela Ziebell
18. Analysis of Transgenic Glycoside Hydrolases Expressed in Plants: T. reesei CBH I and A. cellulolyticus EI
Roman Brunecky, John O. Baker, Hui Wei, Larry E. Taylor, Michael E. Himmel, and Stephen R. Decker
Part III: Lignins and HEmicelluloses
19. Structural Characterization of the Heteroxylans
from Poplar and Switchgrass
Koushik Mazumder, Maria J. Peña, Malcolm A. O'Neill, William S. York
20. Laser Microdissection and Genetic Manipulation Technologies to Probe Lignin Heterogeneity and Configuration in Plant Cell Walls
Oliver R. A. Corea, Chanyoung Ki, Claudia L. Cardenas,
Laurence B. Davin, and Norman G. Lewis
21. Lignin-Degrading Enzyme Activities
Yi-ru Chen, Simo Sarkanen and Yun-Yan Wang
