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Biolistic DNA Delivery: Methods and Protocols

Biolistic DNA Delivery: Methods and Protocols

          
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About the Book

Part I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology

1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun

Caixia Gao and Klaus K. Nielsen

2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery

Shoko Ueki, Shimpei Magori, Benoît Lacroix, and Vitaly Citovsky

3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues

Mulpuri Sujatha and K.B.R.S. Visarada

4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun)

Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen

5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies

Victor Gaba, Moshe Lapidot, and Amit Gal-On

6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun

Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn

Part II: Biolistic DNA Delivery in Nematodes

7. Biolistic Transformation of Caenorhabditis elegans

Meltem Isik and Eugene Berezikov

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans

Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi

Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells

Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples

Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes

David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices

John A. O'Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo

14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun

Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery

Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs

Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter

Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice

Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets

Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models

Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria

Elke S. Bergmann-L


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Product Details
  • ISBN-13: 9781627031097
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Depth: 27
  • Height: 254 mm
  • No of Pages: 415
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm
  • ISBN-10: 162703109X
  • Publisher Date: 29 Oct 2012
  • Binding: Hardback
  • Edition: 1
  • Language: English
  • Returnable: Y
  • Spine Width: 30 mm
  • Weight: 960 gr


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