Part I: The Basics of Atomic Force Microscopy

1. How the Atomic Force Microscope Works

Bruno Torre, Davide Ricci, and Pier Carlo Braga

2. Measurement Methods in Atomic Force Microscopy

Bruno Torre, Claudio Canale, Davide Ricci, and Pier Carlo Braga

3. Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging

Claudio Canale, Bruno Torre, Davide Ricci, and Pier Carlo Braga

Part II: Molecule Imaging

4. Imaging the Spatial Orientation of Subunits within Membrane Receptors by Atomic Force Microscopy

Stewart M Carnally, J. Michael Edwardson, and Nelson P. Barrera

5. High Resolution Imaging of Immunoglobulin G (IgG) Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air

Sergio Santos and Neil H. Thomson

6. Atomic Force Microscopy of Ex vivo Amyloid Fibrils

Claudio Canale, Annalisa Relini, and Alessandra Gliozzi

7. Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy

Clemens M. Franz and Daniel J. Muller

8. Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid

Osamu Hoshi and Tatsuo Ushiki

9. Atomic Force Microscopy of Proteasome Assemblies

Maria Gaczynska and Pawel A. Osmulski

10. Atomic Force Microscopy of Isolated Mitochondria

Bradley E. Layton and M. Brent Boyd

11. Imaging Native Membrane Proteins Using the Atomic Force Microscope

Andreas Engel

Part III: Nanoscale Surface Analysis and Cell Imaging

12. Atomic Force Microscopy Investigation of Viruses

Alexander McPherson and Yurii G. Kuznetsov

13. Determination of the Kinetic On and -Off Rate of Single Virus-Cell Interactions

Christian Rankl, Linda Wildling, Isabel Neundlinger, Ferry Kienberger, Hermann Gruber, Dieter Blaas, and Peter Hinterdorfer

14. Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites

Wanderley de Souza, Gustavo M. Rocha, Kildare Miranda, Paulo M. Bisch, and Gilberto Weissmuller

15. Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy

Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer

16. The Growth Cones of Living Neurons Probed by the Atomic Force Microscope

Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco

17. Highlights on Ultrastructural Pathology of Human Sperm

Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna

18. High-speed Atomic Force Microscopy and Biomolecular Processes

Takayuki Uchihashi and Toshio Ando

Part IV: Non-Topographical Applications (Force-Spectroscopy)

19. Atomic Force Microscopy in Mechanobiology: Measuring Micro-Elastic Heterogeneity of Living Cells

Evren U. Azeloglu and Kevin D. Costa

20. Force-Clamp Measurements of Receptor-Ligand Interactions

Félix Rico, Calvin Chu, and Vincent T. Moy

21. Measuring Cell Adhesion Forces: Theory and Principles

Martin Benoit and Christine Selhuber-Unkel

22. Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces

Ting Cao, Haiyin