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Aspectos teóricos de las pruebas diagnósticas de biología molecular para la identificación de neospora caninum en fetos bovinos de establos del estado de Hidalgo, México

Aspectos teóricos de las pruebas diagnósticas de biología molecular para la identificación de neospora caninum en fetos bovinos de establos del estado de Hidalgo, México

          
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About the Book

Trabajo Universitario del año 2012 en eltema Veterinaria, Nota: 3,99, Idioma: Español, Resumen: Neospora caninum es un protozoario intracelular obligado que pertenece al phylum Apicomplexa, familia Sarcosystidae. Éste parásito, fue confundido con Toxoplasma gondii, debido a sus similitudes estructulares, Thilsted y Dubey identificaron por primera vez a N. caninum en 1988 como agente etiológico de aborto en bovinos. En 1993 se consiguió el primer aislamiento procedente de un feto bovino abortado. Posteriormente, se logró la reproducción de la muerte fetal en vacas gestantes inoculadas experimentalmente. N. caninum se ha detectado en la mayoría de las especies domésticas como los bovinos, quienes ingieren ooquistes excretados por caninos, identificados como hospederos definitivos. Se plantea que la humedad, como es el caso de la zona estudiada, posibilita que los ooquistes expulsados al exterior esporulen y permanezcan en fase infectiva y viables en la tierra, suelos y pastos por varios meses. Esto deviene en una vía de transmisión segura, para el hospedero intermediario. En bovinos gestantes, la ingestión puede conducir a la transmisión transplacentaria exógena o en el caso de infección crónica, a la transmisión endógena debida a la reactivación de quistes que contienen bradizoitos, que, en la mayoría de los casos desencadena el proceso abortivo. Al no haber lesiones macroscópicas patognomónicas, el diagnóstico puede hacerse de diferentes formas. El estudio histopatológico de tejido fetal esencialmente de cerebro se utiliza como prueba de escrutinio, a pesar de su baja sensibilidad; ya que no requiere de una operación costosa. Pero, la aplicación de rtPCR se ha empleado mayoritariamente en tejido del sistema nervioso central, como prueba confirmatoria, por su alta sensibilidad y especificidad, aunque no se ha usado de manera rutinaria en el diagnóstico, básicamente por el alto costo, equipo y experiencia requeridos para desarrollar dicha técnica. En México se ha


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Product Details
  • ISBN-13: 9783656932635
  • Publisher: Grin Verlag Gmbh
  • Binding: Paperback
  • Language: Spanish
  • Returnable: N
  • Spine Width: 2 mm
  • Width: 148 mm
  • ISBN-10: 3656932638
  • Publisher Date: 20 Apr 2015
  • Height: 210 mm
  • No of Pages: 34
  • Series Title: Spanish
  • Weight: 59 gr

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Aspectos teóricos de las pruebas diagnósticas de biología molecular para la identificación de neospora caninum en fetos bovinos de establos del estado de Hidalgo, México
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Aspectos teóricos de las pruebas diagnósticas de biología molecular para la identificación de neospora caninum en fetos bovinos de establos del estado de Hidalgo, México
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