Part I: General Overview
1. Worldwide Clinical Demand for Antibiotics: Is It a Real Countdown?
Carlos Barreiro and José-Luis Barredo
Part II: Antibiotic Screening
2. Collection, Extraction, and In Vitro Antibacterial Evaluation of Plants Used in Traditional Medicine
François Chassagne and Cassandra Leah Quave
3. Microbial Isolation and Characterization of New Antibiotic-Producer Strains from Decayed Wood
Óscar Velasco-Rodríguez, Mariana Fil, Laura García-Calvo, Katarina Kosalková, and Carlos Barreiro
4. Characterization of Extremophilic Actinomycetes Strains as Sources of Antimicrobial Agents
Diyora Abdukhakimova, Zhannur Markhametova, Saikal Shamkeeva, Ainur Zhulamanova, Lyudmila Trenozhnikova, Vladimir Berezin, and Azliyati Azizan
5. Dilution-To-Extinction Platform for the Isolation of Marine Bacteria Producing Antitumor Compounds Xulio Benítez, Jesús García, Elena G. González, and Fernando de la Calle
Part III: Methodologies
6. Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species Jan Kormanec, Bronislava Rezuchova, and Renata Novakova
7. Antibacterial Assay in a Screening Project
Francisco Romero and Antonio Fernández
8. Screening of Antibiotic Gene Clusters in Microorganisms Isolated from Wood
Mariana Fil, Óscar Velasco-Rodríguez, Laura García-Calvo, Alberto Sola-Landa, and Carlos Barreiro
9. Methods in Screening Antiviral Drugs Against Enterovirus 71
Nadia Abubakar Omar Abubakar Bajaber and Babu Ramanathan
10. High-Efficiency Electroporation for Genetic Improvement of Fungal Strains
Jessica Cruz-Ramón, Francisco J. Fernández, and Francisco Fierro
11. Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC
Katarina Kosalková, Isabel-Clara Sánchez-Orejas, Laura Cueto, and Carlos García-Estrada
12. Screening Fermentation and Extract Generation
Francisco Romero and Antonio Fernández
13. A Simple Antifungal Assay for Testing Actinomycetes and Other Microbial Extracts
María A. Vinuesa and Antonio Fernández
14. A User Guide for the Identification of New RiPP Biosynthetic Gene Clusters Using a RiPPER-Based Workflow
Alaster D. Moffat, Javier Santos-Aberturas, Govind Chandra, and Andrew W. Truman
15. Novel Methods to Identify Oxidative Stress-Producing Antibiotics
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