Part I. In silico Methods

1. Use of IMGT(R) Databases and Tools for Antibody Engineering and Humanization

Marie-Paule Lefranc, François Ehrenmann, Chantal Ginestoux, Véronique Giudicelli, and Patrice Duroux

2. Computer-Assisted Modeling of Antibody Variable Domains

Oscar H. P. Ramos

Part II. Generation of Diversity

3. Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells

Lars Toleikis and André Frenzel

4. Human in Cell scFv Library from Infiltrating B Cell

Sylvie Peraldi-Roux

5. Construction of Human Naive Antibody Gene Libraries

Michael Hust, André Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dübel

6. Synthetic Customized scFv Libraries

Gautier Robin and Pierre Martineau

7. Selection of Stable scFv Antibodies by Phage Display

Eeva-Christine Brockmann

8. Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs

Cécile Vincke, Carlos Gutiérez, Ulrich Wernery, Nick Devoogdt, Gholamreza Hassanzadeh-Ghassabeh, and Serge Muyldermans

9. Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires

Mischa Roland Müller, Ronan O'Dwyer, Marina Kovaleva, Fiona Rudkin, Helen Dooley, and Caroline Jane Barelle

10. Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis

Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ

Part IV. Selections of Lead Candidate

11. Phage Display and Selections on Purified Antigens

Julie Matz and Patrick Chames

12. Phage Display and Selections on Cells

Klervi Even-Desrumeaux and Patrick Chames

13. Humanization by CDR Grafting and Specificity-Determining Residue Grafting

Jin Hong Kim and Hyo Jeong Hong

14. Humanization by Guided Selections

Sang Jick Kim and Hyo Jeong Hong

15. Selection of Antibody Fragments by Yeast Display

Nathalie Scholler

16. Evolution of Antibodies in vitro by Ribosome Display

Bryan M. Edwards and Mingyue He

17. Mammalian Cell Display of Full Length IgG

Chen Zhou and Wenyan (David) Shen

Part V. Production of Recombinant Antibodies

18. Production of Antibody Fragments in Escherichia coli

Tomohisa Katsuda, Hiroyuki Sonoda, and Hideki Yamaji

19. Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris

Steve Schoonooghe, Jannick Leoen, and Jurgen Haustraete

20. Monoclonal Antibody Expression in Mammalian Cells

Richard (Yi) Zhang and Wenyan (David) Shen

21. Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells

Daniel X. Johansson, Thomas Krey, and Oskar Andersson

22. Production of Antibody Fragments Using the Baculovirus-Insect Cell System

Takanori Furuta, Takafumi Ogawa, and Hideki Yamaji

23. Transient and Stable Expression of Antibodies in Nicotiana Species

J. Christopher Hall, Michael D. McLean and Freydoun Garabagi

Part VI. Variable Domain Optimization

24. Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance

Stephen Hearty, Paul Leonard, and Richard O'Kennedy

25. Affinity Determination of Biotinylated Antibodies by Flow Cytometry

Klervi Even-Desrumeaux and Patrick Chames

26. Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput Screenin