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Analyse des médicaments antipaludiques dans le plasma humain par LC-MS/MS

Analyse des médicaments antipaludiques dans le plasma humain par LC-MS/MS

          
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About the Book

La méthode présente une procédure d'extraction efficace basée sur l'extraction liquide-liquide (ELL) qui permet une récupération élevée et constante des deux analytes. L'effet de matrice a été évalué de manière approfondie en estimant les facteurs de matrice normalisés par l'IS, ainsi que par la perfusion post-colonne de l'analyte. La méthode LC-MS/MS développée pour la quantification de l'ART, du DHA, du LF et du DLF dans le plasma humain a été entièrement validée conformément aux directives de la FDA américaine. La méthode proposée est sensible pour les deux analytes par rapport à toutes les autres méthodes présentées soit en tant qu'analyte unique soit en combinaison dans différentes matrices biologiques. La méthode offre des avantages significatifs par rapport à celles précédemment rapportées, en termes de faible besoin en échantillons pour l'analyse, de simplicité de la procédure d'extraction et de temps d'analyse global. L'absence d'interférence de la matrice est effectivement démontrée par la perfusion post-colonne et par les valeurs de précision (% CV) pour les pentes calculées des courbes d'étalonnage. La méthode validée a montré des données acceptables pour tous les paramètres de validation, avec une sensibilité et une sélectivité adéquates pour leur quantification simultanée dans un contexte clinique.


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Product Details
  • ISBN-13: 9786205530160
  • Publisher: KS Omniscriptum Publishing
  • Publisher Imprint: Editions Notre Savoir
  • Height: 229 mm
  • No of Pages: 108
  • Spine Width: 7 mm
  • Width: 152 mm
  • ISBN-10: 6205530163
  • Publisher Date: 20 May 2023
  • Binding: Paperback
  • Language: French
  • Returnable: N
  • Weight: 172 gr

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Analyse des médicaments antipaludiques dans le plasma humain par LC-MS/MS
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